Cloned (Comment) | Organism |
---|---|
expression of the His-tagged enzyme in Escherichia coli | Burkholderia cepacia |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | no activity is detected when 2 mM EDTA is added to the dialysis buffer | Burkholderia cepacia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.01 | - |
D-mannose 6-phosphate | pH 7.6, 5 mM Mg2+, temperature not specified in the publication | Burkholderia cepacia |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | divalent ions are necessary for PMI maximal activity in decreasing order of activation: Ca2+, Mn2+, Mg2+, Co2+, Ni2+ | Burkholderia cepacia | |
Co2+ | divalent ions are necessary for PMI maximal activity in decreasing order of activation: Ca2+, Mn2+, Mg2+, Co2+, Ni2+ | Burkholderia cepacia | |
Mg2+ | divalent ions are necessary for PMI maximal activity in decreasing order of activation: Ca2+, Mn2+, Mg2+, Co2+, Ni2+ | Burkholderia cepacia | |
Mn2+ | divalent ions are necessary for PMI maximal activity in decreasing order of activation: Ca2+, Mn2+, Mg2+, Co2+, Ni2+ | Burkholderia cepacia | |
Ni2+ | divalent ions are necessary for PMI maximal activity in decreasing order of activation: Ca2+, Mn2+, Mg2+, Co2+, Ni2+ | Burkholderia cepacia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
SDS-PAGE | Burkholderia cepacia |
55300 | - |
calculated from sequence | Burkholderia cepacia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | Q06XM8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Burkholderia cepacia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Mannose 6-phosphate | - |
Burkholderia cepacia | D-Fructose 6-phosphate | - |
? | |
D-Mannose 6-phosphate | - |
Burkholderia cepacia IST408 | D-Fructose 6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BceA | - |
Burkholderia cepacia |
Phosphomannose isomerase | - |
Burkholderia cepacia |
PMI | - |
Burkholderia cepacia |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19.4 | - |
D-mannose 6-phosphate | pH 7.6, 5 mM Mg2+, temperature not specified in the publication | Burkholderia cepacia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Burkholderia cepacia |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Burkholderia cepacia | calculated from sequence | - |
6.03 |
General Information | Comment | Organism |
---|---|---|
malfunction | the lack of a functional bceA gene does not affect exopolysaccharide production yield in a non-polar insertion bceA mutant. The in silico search for putative bceA homologues reveals the presence of 2-5 bceA orthologues in the Burkholderia genomes available. This suggests that in Burkholderia cepacia IST408 putative bceA functional homologues may compensate the bceA mutation | Burkholderia cepacia |
physiological function | the enzyme is involved in cepacian production yield and rheological properties and in the size of biofilms formed | Burkholderia cepacia |