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Literature summary for 5.3.1.8 extracted from
- Characterization of a mannose-6-phosphate isomerase from Geobacillus thermodenitrificans that converts monosaccharides (2009), Biotechnol. Lett., 31, 1273-1278.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli strain ER2566 | Geobacillus thermodenitrificans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | strong inhibition | Geobacillus thermodenitrificans |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | enzyme kinetics with D-lyxose and L-ribose, overview | Geobacillus thermodenitrificans |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Geobacillus thermodenitrificans | |
| Co2+ | activates, best activator metal ion | Geobacillus thermodenitrificans | |
| Cu2+ | activates | Geobacillus thermodenitrificans | |
| Fe2+ | activates | Geobacillus thermodenitrificans | |
| Mg2+ | activates | Geobacillus thermodenitrificans | |
| Mn2+ | activates | Geobacillus thermodenitrificans | |
| additional information | the enzyme requires divalent cations for activity | Geobacillus thermodenitrificans | |
| Ni2+ | activates | Geobacillus thermodenitrificans | |
| Zn2+ | activates poorly | Geobacillus thermodenitrificans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
gel filtration, recombinant enzyme | Geobacillus thermodenitrificans |
| 36452 | - |
1 * 36000, recombinant enzyme, SDS-PAGE, 1 * 36452, sequence calculation | Geobacillus thermodenitrificans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Mannose 6-phosphate | Geobacillus thermodenitrificans | - |
D-Fructose 6-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus thermodenitrificans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme 8.7fold from Escherichia coli strain ER2566 | Geobacillus thermodenitrificans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 890 | - |
purified recombinant enzyme | Geobacillus thermodenitrificans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Lyxose | 38% conversion yield after 3 h | Geobacillus thermodenitrificans | D-Xylulose | - |
? | |
| D-Mannose 6-phosphate | - |
Geobacillus thermodenitrificans | D-Fructose 6-phosphate | - |
r | |
| L-ribose | 29% conversion yield after 3 h | Geobacillus thermodenitrificans | L-ribulose | - |
? | |
| additional information | enzyme substrate specificity with diverse monosaccharide aldoses and ketoses, overview | Geobacillus thermodenitrificans | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 36000, recombinant enzyme, SDS-PAGE, 1 * 36452, sequence calculation | Geobacillus thermodenitrificans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GTMpi | - |
Geobacillus thermodenitrificans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Geobacillus thermodenitrificans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 80 | - |
Geobacillus thermodenitrificans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermal inactivation of GTMpi follows first-order kinetics | Geobacillus thermodenitrificans |
| 60 | - |
enzyme half-life is 388 h | Geobacillus thermodenitrificans |
| 65 | - |
enzyme half-life is 73 h | Geobacillus thermodenitrificans |
| 70 | - |
enzyme half-life is 27 h | Geobacillus thermodenitrificans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Geobacillus thermodenitrificans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | - |
Geobacillus thermodenitrificans |
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