Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli ER2566 cells | Trypanosoma cruzi |
Crystallization (Comment) | Organism |
---|---|
RpiB (wild type or C69A mutant enzyme), sitting drop vapor diffusion method, using 0.5% (v/v) Jeffamine ED-2001, 0.1 M HEPES, 1.1 M Na-malonate (pH 7.0) for the wild type enzyme in complex with phosphate, or 0.8 M Na?K phosphate (pH 8.2) for the mutant enzyme C69A in complex with phosphate, or 20% (w/v) poly(ethyleneglycol) 550 monomethyl ether, 0.1 M NaCl, 0.1 M bicine (pH 9.0) for the wild type enzyme in complex with D-ribose 5-phosphate, or 20% (w/v) poly(ethylene glycol) 6000, 0.2 M ammonium chloride, 0.1 M Tris-HCl (pH 8.0) for wild type enzyme in complex with 4-phospho-D-erythronohydroxamic acid, or 20% (w/v) poly(ethylene glycol) 3350, 0.2 M sodium acetate, 0.1 M bis-Tris propane (pH 8.5) for mutant enzyme C69A in complex with allose 6-phosphate | Trypanosoma cruzi |
Protein Variants | Comment | Organism |
---|---|---|
C69A | inactive | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-Allose 6-phosphate | weak competitive inhibitor | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
D-ribulose 5-phosphate | in 50 mM Tris-HCl (pH 7.6), 150 mM NaCl and 5 mM MESNA, at 30°C | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | Q4CQE2 | - |
- |
Trypanosoma cruzi CL Brener | Q4CQE2 | - |
- |
Purification (Comment) | Organism |
---|---|
Superdex 75 gel filtration | Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 5-phosphate | - |
Trypanosoma cruzi | D-ribose 5-phosphate | - |
? | |
D-ribulose 5-phosphate | - |
Trypanosoma cruzi CL Brener | D-ribose 5-phosphate | - |
? | |
additional information | the enzyme cannot isomerizes D-allose 6-phosphate | Trypanosoma cruzi | ? | - |
? | |
additional information | the enzyme cannot isomerizes D-allose 6-phosphate | Trypanosoma cruzi CL Brener | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | x-ray crystallography | Trypanosoma cruzi |
Synonyms | Comment | Organism |
---|---|---|
ribose-5-phosphate isomerase | - |
Trypanosoma cruzi |
RpiB | - |
Trypanosoma cruzi |
type B ribose 5-phosphate isomerase | - |
Trypanosoma cruzi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 8 | D-ribulose 5-phosphate | in 50 mM Tris-HCl (pH 7.6), 150 mM NaCl and 5 mM MESNA, at 30°C | Trypanosoma cruzi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
D-Allose 6-phosphate | in 50 mM Tris-HCl (pH 7.6), 150 mM NaCl and 5 mM MESNA, at 30°C | Trypanosoma cruzi |