Cloned (Comment) | Organism |
---|---|
separation of the 2 domains of the indole-3-glycerol-phosphate synthetase/PRA isomerase complex on the gene level, expression of monofunctional PRAI[ML256-452] in Escherichia coli W3110 trpR, deltatrp EA2 | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | product inhibition | Escherichia coli | |
additional information | not inhibited by indoleglycerol phosphate | Escherichia coli | |
reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | competitive inhibitor | Escherichia coli | |
reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | competitive inhibitor | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0047 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | 25°C, monomeric domain PRAI[ML256-452] | Escherichia coli | |
0.0049 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | 25°C, bifunctional IGPS:PRAI | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
- |
Saccharomyces cerevisiae |
23000 | - |
monomeric domain PRAI[ML256-452], gel filtration and analytical ultracentrifugation | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-(5-phospho-beta-D-ribosyl)anthranilate | Escherichia coli | involved in tryptophan biosynthesis from chorismate | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
N-(5-phospho-beta-D-ribosyl)anthranilate | Saccharomyces cerevisiae | involved in tryptophan biosynthesis from chorismate | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
N-(5-phospho-beta-D-ribosyl)anthranilate | Saccharomyces cerevisiae xPRAI | involved in tryptophan biosynthesis from chorismate | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
bifunctional indole-3-glycerol-phosphate synthetase/PRA isomerase complex | - |
Saccharomyces cerevisiae | - |
xPRAI | - |
Saccharomyces cerevisiae xPRAI | - |
xPRAI | - |
Purification (Comment) | Organism |
---|---|
monomeric domain PRAI[ML256-452] and bifunctional IGPS:PRAI | Escherichia coli |
recombinant xPRAI, expressed in Escherichia coli | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
98.7 | - |
monofunctional domain PRAI[ML256-452] | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-(5-phospho-beta-D-ribosyl)anthranilate | - |
Escherichia coli | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
N-(5-phospho-beta-D-ribosyl)anthranilate | - |
Saccharomyces cerevisiae | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
N-(5-phospho-beta-D-ribosyl)anthranilate | involved in tryptophan biosynthesis from chorismate | Escherichia coli | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
N-(5-phospho-beta-D-ribosyl)anthranilate | involved in tryptophan biosynthesis from chorismate | Saccharomyces cerevisiae | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
N-(5-phospho-beta-D-ribosyl)anthranilate | - |
Saccharomyces cerevisiae xPRAI | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
N-(5-phospho-beta-D-ribosyl)anthranilate | involved in tryptophan biosynthesis from chorismate | Saccharomyces cerevisiae xPRAI | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PRAI[ML256-452] | engineered monofunctional variant of IGPS:PRAI, excised monomeric domain | Escherichia coli |
PRAI[ML256-452] | engineered monofunctional variant of IGPS:PRAI, excised monomeric domain | Saccharomyces cerevisiae |
xPRAI | monomeric variant of PRAI from Saccharomyces cerevisiae expressed in Escherichia coli | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
32 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | 25°C, monomeric domain PRAI[ML256-452] | Escherichia coli | |
40 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | 25°C | Escherichia coli | |
40 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | bifunctional IGPS:PRAI | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0022 | - |
reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | recombinant xPRAI, expressed in Escherichia coli | Saccharomyces cerevisiae | |
0.0065 | - |
reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | 25°C, bifunctional IGPS:PRAI | Escherichia coli | |
0.0068 | - |
reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | 25°C, monomeric domain PRAI[ML256-452] | Escherichia coli |