Cloned (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Crystallization (Comment) | Organism |
---|---|
crystal structures with the inhibitor FK506 bound to the FK506-binding domain, and with 15-residue-long peptides bound to either one or both domains. Substrates bind in a highly adaptable fashion to the insert-in-flap chaperone domain through beta-strand augmentation, and can bind to the FK506-binding domain | Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
A78G | 52% of wild-type activity | Thermus thermophilus |
D23A | 78% of wild-type activity | Thermus thermophilus |
F128A | 35% of wild-type activity | Thermus thermophilus |
H119A | 84% of wild-type activity | Thermus thermophilus |
I37G | 50% of wild-type activity | Thermus thermophilus |
M96A | 39% of wild-type activity | Thermus thermophilus |
additional information | a mutant lacking the insert-in-flap chaperone domain shoiws 58% of wild-type activity | Thermus thermophilus |
N35A | 71% of wild-type activity | Thermus thermophilus |
Q94A | 98% of wild-type activity | Thermus thermophilus |
Y13F | 92% of wild-type activity | Thermus thermophilus |
Y63A | 21% of wild-type activity | Thermus thermophilus |
Y63F | 76% of wild-type activity | Thermus thermophilus |
Y92A | 36% of wild-type activity | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
FK506 | - |
Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
TRYWNAKMK-(cis)-PFIFGA | wild-type, pH 7.5, temperature not specified in the publication | Thermus thermophilus |
Organic Solvent | Comment | Organism |
---|---|---|
dimethyl sulfoxide | 5%, only minor effects on binding of peptide substrates | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q5SLE7 | - |
- |
Thermus thermophilus DSM 579 | Q5SLE7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | peptide substrates derived from ribosomal proteins S2 and S3 display a dual binding mode with both a high- and a low-affinity binding site. Peptide TRYWNPKMKPFIFGA from protein S2 binds to both the insert-in-flap and FK506-binding domains | Thermus thermophilus | ? | - |
? | |
additional information | peptide substrates derived from ribosomal proteins S2 and S3 display a dual binding mode with both a high- and a low-affinity binding site. Peptide TRYWNPKMKPFIFGA from protein S2 binds to both the insert-in-flap and FK506-binding domains | Thermus thermophilus DSM 579 | ? | - |
? | |
N-succinyl-Ala-Leu-(cis)-Pro-Phe-4-nitroanilide | - |
Thermus thermophilus | N-succinyl-Ala-Leu-(trans)-Pro-Phe-4-nitroanilide | - |
r | |
N-succinyl-Ala-Leu-(cis)-Pro-Phe-4-nitroanilide | - |
Thermus thermophilus DSM 579 | N-succinyl-Ala-Leu-(trans)-Pro-Phe-4-nitroanilide | - |
r | |
TRYWNAKMK-(cis)-PFIFGA | - |
Thermus thermophilus | TRYWNAKMK-(trans)-PFIFGA | - |
r | |
TRYWNAKMK-(cis)-PFIFGA | - |
Thermus thermophilus DSM 579 | TRYWNAKMK-(trans)-PFIFGA | - |
r |
Synonyms | Comment | Organism |
---|---|---|
SLyD | - |
Thermus thermophilus |
TTHA0346 | - |
Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
70000 | - |
TRYWNAKMK-(cis)-PFIFGA | wild-type, pH 7.5, temperature not specified in the publication | Thermus thermophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
850 | - |
N-succinyl-Ala-Leu-(cis)-Pro-Phe-4-nitroanilide | mutant lacking the insert-in-flap domain, pH 7.5, temperature not specified in the publication | Thermus thermophilus | |
1470 | - |
N-succinyl-Ala-Leu-(cis)-Pro-Phe-4-nitroanilide | wild-type, pH 7.5, temperature not specified in the publication | Thermus thermophilus | |
248000 | - |
TRYWNAKMK-(cis)-PFIFGA | mutant lacking the insert-in-flap domain, pH 7.5, temperature not specified in the publication | Thermus thermophilus | |
368000 | - |
TRYWNAKMK-(cis)-PFIFGA | wild-type, pH 7.5, temperature not specified in the publication | Thermus thermophilus |