BRENDA - Enzyme Database show
show all sequences of 5.1.3.6

The biosynthesis of UDP-galacturonic acid in plants. Functional cloning and characterization of Arabidopsis UDP-D-glucuronic acid 4-epimerase

Gu, X.; Bar-Peled, M.; Plant Physiol. 136, 4256-4264 (2004)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
no recovery of active recombinant enzyme when full-length AtUGlcAE1 is expressed in Escherichia coli due to the hydrophobic domain that results in un- or misfolded protein in inclusion bodies within Escherichia coli. Recombinant AtUGlcAE1DELTA1-64, lacking the putative transmembrane domain, can be expressed in Escherichia coli
Arabidopsis thaliana
General Stability
General Stability
Organism
KCl or NaCl, above 300 mM, completly inactivates the enzyme, when stored at -20°C for 2 weeks
Arabidopsis thaliana
Inhibitors
Inhibitors
Commentary
Organism
Structure
ADP-Ara
2 mM, about 55% inhibition
Arabidopsis thaliana
KCl
above 300 mM, inhibits activity of recombinant AtUGlcAE1DELTA1-64
Arabidopsis thaliana
NaCl
above 300 mM, inhibits activity of recombinant AtUGlcAE1DELTA1-64
Arabidopsis thaliana
UDP
2 mM, about 60% inhibition
Arabidopsis thaliana
UDP-Xyl
2 mM, about 60% inhibition
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.72
-
UDP-D-glucuronate
-
Arabidopsis thaliana
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43500
-
2 * 43500
Arabidopsis thaliana
88000
-
recombinant AtUGlcAE1DELTA1-64, gel filtration
Arabidopsis thaliana
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
O22141
-
-
Purification (Commentary)
Commentary
Organism
partial, AtUGlcAE1DELTA1-64 expressed in Escherichia coli
Arabidopsis thaliana
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
stem
-
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-D-glucuronate
recombinant AtUGlcAE1DELTA1-64 favors the formation of UDP-D-galacturonate over UDP-glucuronate
663134
Arabidopsis thaliana
UDP-D-galacturonate
-
-
-
r
Subunits
Subunits
Commentary
Organism
dimer
2 * 43500
Arabidopsis thaliana
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
42
recombinant AtUGlcAE1DELTA1-64
Arabidopsis thaliana
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
24
-
UDP-glucuronate
-
Arabidopsis thaliana
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
7.6
recombinant AtUGlcAE1DELTA1-64
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
no recovery of active recombinant enzyme when full-length AtUGlcAE1 is expressed in Escherichia coli due to the hydrophobic domain that results in un- or misfolded protein in inclusion bodies within Escherichia coli. Recombinant AtUGlcAE1DELTA1-64, lacking the putative transmembrane domain, can be expressed in Escherichia coli
Arabidopsis thaliana
General Stability (protein specific)
General Stability
Organism
KCl or NaCl, above 300 mM, completly inactivates the enzyme, when stored at -20°C for 2 weeks
Arabidopsis thaliana
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ADP-Ara
2 mM, about 55% inhibition
Arabidopsis thaliana
KCl
above 300 mM, inhibits activity of recombinant AtUGlcAE1DELTA1-64
Arabidopsis thaliana
NaCl
above 300 mM, inhibits activity of recombinant AtUGlcAE1DELTA1-64
Arabidopsis thaliana
UDP
2 mM, about 60% inhibition
Arabidopsis thaliana
UDP-Xyl
2 mM, about 60% inhibition
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.72
-
UDP-D-glucuronate
-
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43500
-
2 * 43500
Arabidopsis thaliana
88000
-
recombinant AtUGlcAE1DELTA1-64, gel filtration
Arabidopsis thaliana
Purification (Commentary) (protein specific)
Commentary
Organism
partial, AtUGlcAE1DELTA1-64 expressed in Escherichia coli
Arabidopsis thaliana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
stem
-
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-D-glucuronate
recombinant AtUGlcAE1DELTA1-64 favors the formation of UDP-D-galacturonate over UDP-glucuronate
663134
Arabidopsis thaliana
UDP-D-galacturonate
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 43500
Arabidopsis thaliana
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
42
recombinant AtUGlcAE1DELTA1-64
Arabidopsis thaliana
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
24
-
UDP-glucuronate
-
Arabidopsis thaliana
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
7.6
recombinant AtUGlcAE1DELTA1-64
Arabidopsis thaliana
Other publictions for EC 5.1.3.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
716581
Wojtasik
The changes in pectin metaboli ...
Linum usitatissimum
Plant Physiol. Biochem.
49
862-872
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
716216
Pang
Comparative proteomics indicat ...
Arabidopsis thaliana, Gossypium hirsutum
Mol. Cell. Proteomics
9
2019-2033
2010
-
-
2
-
-
-
-
-
-
-
-
-
-
4
-
-
2
-
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
700488
Siddique
Myo-inositol oxygenase genes a ...
Arabidopsis thaliana
New Phytol.
184
457-472
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
704357
Gu
Enzymatic characterization and ...
Arabidopsis thaliana, Oryza sativa, Zea mays
J. Biochem.
146
527-534
2009
-
-
3
-
1
-
6
4
-
-
7
5
-
12
-
-
3
-
-
-
-
1
5
3
3
-
3
4
3
1
-
-
10
-
-
-
-
5
-
-
1
-
-
10
10
4
-
-
8
5
-
-
-
5
-
-
-
1
5
4
5
-
3
4
4
1
-
-
-
-
-
-
-
-
661739
Usadel
Identification and characteriz ...
Arabidopsis thaliana
FEBS Lett.
569
327-331
2004
-
-
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
8
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
14
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
663125
Molhoj
The biosynthesis of D-galactur ...
Arabidopsis thaliana
Plant Physiol.
135
1221-1230
2004
-
-
1
-
-
1
2
1
1
-
-
-
-
4
-
-
-
-
-
6
-
1
6
-
-
-
-
-
1
1
-
2
-
-
-
-
-
1
2
-
-
1
-
2
-
1
1
-
-
-
-
-
-
-
-
11
-
1
6
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
663134
Gu
The biosynthesis of UDP-galact ...
Arabidopsis thaliana
Plant Physiol.
136
4256-4264
2004
-
-
1
-
-
1
5
1
-
-
2
-
-
3
-
-
1
-
-
4
-
-
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
5
-
1
-
-
2
-
-
-
-
1
-
4
-
-
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-
653259
Munoz
First molecular characterizati ...
Streptococcus pneumoniae
Mol. Microbiol.
31
703-713
1999
-
-
1
-
-
-
12
1
1
-
2
1
-
3
-
-
1
-
-
-
-
-
2
1
1
-
4
-
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
12
-
1
1
-
2
1
-
-
-
1
-
-
-
-
2
1
1
-
4
-
1
1
-
-
-
-
-
-
-
-
2403
Bolwell
-
Decrease of polygalacturonic a ...
Acer pseudoplatanus
Phytochemistry
24
699-702
1985
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2401
Dalessandro
Changes in enzymic activities ...
Abies grandis, Pinus sylvestris
Biochem. J.
162
281-288
1977
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
4
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
4
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2405
Maitra
The mechanism of uridine dipho ...
Dolichospermum flos-aquae
J. Biol. Chem.
249
3075-3078
1974
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2406
Gaunt
Uridine diphosphate galacturon ...
Dolichospermum flos-aquae
J. Biol. Chem.
249
2366-2372
1974
-
-
-
-
-
-
14
1
-
-
1
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
14
-
1
-
-
1
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
2407
Davidson
-
UDP-D-galacturonic acid 4-epim ...
Raphanus sativus
Methods Enzymol.
8
276-277
1966
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2408
Feingold
The 4-epimerization and decarb ...
Vigna radiata var. radiata
J. Biol. Chem.
235
910-913
1960
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-