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Literature summary for 5.1.3.37 extracted from
- The cell-wall active mannuronan C5-epimerases in the model brown alga Ectocarpus From gene context to recombinant protein (2016), Glycobiology, 26, 973-983 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene MEP13, genetic structure, phylogenetic tree, recombinant expression of the codon-optimized His-tagged catalytic domain, MEP13-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL in inclusion bodies. The usage of Escherichia coli strain Rosettagami2(DE3)pLysS produces larger and more significant inclusion bodies than Escherichia coli strain BL21CodonPlus(DE3)RIPL | Ectocarpus siliculosus |
| gene MEP18, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP18-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL | Ectocarpus siliculosus |
| gene MEP2, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP2-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL | Ectocarpus siliculosus |
| gene MEP21, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP21-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL | Ectocarpus siliculosus |
| gene MEP25, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP25-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL | Ectocarpus siliculosus |
| gene MEP26, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP26-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL | Ectocarpus siliculosus |
| gene MEP27, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP27-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL | Ectocarpus siliculosus |
| gene MEP28, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP28-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL | Ectocarpus siliculosus |
| gene MEP29, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP29-C5, in Escherichia coli strain BL 21CodonPlus(DE3)RIPL | Ectocarpus siliculosus |
| gene MEP4, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP4-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL | Ectocarpus siliculosus |
| gene MEP6, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP6-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL | Ectocarpus siliculosus |
| gene MEP7, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP7-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL | Ectocarpus siliculosus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Ectocarpus siliculosus | effect of ManC5-Es on alginate structures, overview. Alginate in brown algae is first formed as a polysaccharide chain containing mannuronic acid residues only. These are subsequently transformed by the ManC5-E into guluronic acid residues, generating distinct patterns arranged in regions of MM-, GG- and MG-blocks (beta-D-mannuronic acid (M) and alpha-L-guluronic acid (G) residues). Patterns containing large stretches of adjacent guluronic acid residues (GG-blocks) form structured interchain associations in the presence of Ca2+ ions. These interchain junctions have the so-called egg-box conformation and are responsible for the gelling properties of alginate and cell-wall strengthening | ? | - |
? |  |
| additional information | Ectocarpus siliculosus | effect of ManC5-Es on alginate structures, overview. Alginate in brown algae is first formed as a polysaccharide chain containing mannuronic acid residues only. These are subsequently transformed by the ManC5-E into guluronic acid residues, generating distinct patterns arranged in regions of MM-, GG- and MG-blocks (beta-D-mannuronic acid (M) and alpha-L-guluronic acid (G) residues). Patterns containing large stretches of adjacent guluronic acid residues (GG-blocks) form structured interchain associations in the presence of Ca2+ ions. These interchain junctions have the socalled egg-box conformation and are responsible for the gelling properties of alginate and cell-wall strengthening | ? | - |
? | |
| [mannuronan]-beta-D-mannuronate | Ectocarpus siliculosus | - |
[alginate]-alpha-L-guluronate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ectocarpus siliculosus | - |
- |
- |
| Ectocarpus siliculosus | D7FSX3 | MEP28 | - |
| Ectocarpus siliculosus | D7FWW1 | MEP25 | - |
| Ectocarpus siliculosus | D7FXE4 | MEP7 | - |
| Ectocarpus siliculosus | D7G1G1 | MEP21 | - |
| Ectocarpus siliculosus | D7G257 | MEP18 | - |
| Ectocarpus siliculosus | D7G340 | MEP6 | - |
| Ectocarpus siliculosus | D7G651 AND D7G652 | MEP27 central and C-terminal | - |
| Ectocarpus siliculosus | D7G8D9 | MEP13 | - |
| Ectocarpus siliculosus | D8LC73 | MEP4 | - |
| Ectocarpus siliculosus | D8LD45 | MEP2 | - |
| Ectocarpus siliculosus | D8LL67 | MEP26 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged catalytic domain, MEP13-C5, solubilized from Escherichia coli strain BL21 CodonPlus(DE3)RIPL inclusion bodies | Ectocarpus siliculosus |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| recombinant His-tagged catalytic domain, MEP13-C5, from Escherichia coli strain BL21 CodonPlus(DE3)RIPL inclusion bodies, the protein is successfully refolded using an on-column refolding procedure | Ectocarpus siliculosus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| gametophyte | - |
Ectocarpus siliculosus | - |
| additional information | microarray analysis of the abundance of ManC5-E transcripts in Ectocarpus sporophytes versus gametophytes, overview | Ectocarpus siliculosus | - |
| sporophyte | - |
Ectocarpus siliculosus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | effect of ManC5-Es on alginate structures, overview. Alginate in brown algae is first formed as a polysaccharide chain containing mannuronic acid residues only. These are subsequently transformed by the ManC5-E into guluronic acid residues, generating distinct patterns arranged in regions of MM-, GG- and MG-blocks (beta-D-mannuronic acid (M) and alpha-L-guluronic acid (G) residues). Patterns containing large stretches of adjacent guluronic acid residues (GG-blocks) form structured interchain associations in the presence of Ca2+ ions. These interchain junctions have the so-called egg-box conformation and are responsible for the gelling properties of alginate and cell-wall strengthening | Ectocarpus siliculosus | ? | - |
? | |
| additional information | effect of ManC5-Es on alginate structures, overview. Alginate in brown algae is first formed as a polysaccharide chain containing mannuronic acid residues only. These are subsequently transformed by the ManC5-E into guluronic acid residues, generating distinct patterns arranged in regions of MM-, GG- and MG-blocks (beta-D-mannuronic acid (M) and alpha-L-guluronic acid (G) residues). Patterns containing large stretches of adjacent guluronic acid residues (GG-blocks) form structured interchain associations in the presence of Ca2+ ions. These interchain junctions have the socalled egg-box conformation and are responsible for the gelling properties of alginate and cell-wall strengthening | Ectocarpus siliculosus | ? | - |
? | |
| additional information | subatrate specificity and change of blockiness of the active recombinant His-tagged catalytic domain is analyzed by NMR study, overview | Ectocarpus siliculosus | ? | - |
? | |
| [mannuronan]-beta-D-mannuronate | - |
Ectocarpus siliculosus | [alginate]-alpha-L-guluronate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | purified recombinant His-tagged catalytic domain peptide mass fingerprinting | Ectocarpus siliculosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ManC5-E | - |
Ectocarpus siliculosus |
| mannuronan C5-epimerase | - |
Ectocarpus siliculosus |
| MEP13 | - |
Ectocarpus siliculosus |
| MEP13-C5 | - |
Ectocarpus siliculosus |
| MEP18 | - |
Ectocarpus siliculosus |
| MEP18-C5 | - |
Ectocarpus siliculosus |
| Mep2 | - |
Ectocarpus siliculosus |
| MEP2-C5 | - |
Ectocarpus siliculosus |
| MEP21 | - |
Ectocarpus siliculosus |
| MEP21-C5 | - |
Ectocarpus siliculosus |
| MEP25 | - |
Ectocarpus siliculosus |
| MEP25-C5 | - |
Ectocarpus siliculosus |
| MEP26 | - |
Ectocarpus siliculosus |
| MEP26-C5 | - |
Ectocarpus siliculosus |
| MEP27 | - |
Ectocarpus siliculosus |
| MEP27-C5 | - |
Ectocarpus siliculosus |
| MEP28 | - |
Ectocarpus siliculosus |
| MEP28-C5 | - |
Ectocarpus siliculosus |
| MEP29 | - |
Ectocarpus siliculosus |
| MEP29-C5 | - |
Ectocarpus siliculosus |
| Mep4 | - |
Ectocarpus siliculosus |
| MEP4-C5 | - |
Ectocarpus siliculosus |
| Mep6 | - |
Ectocarpus siliculosus |
| MEP6-C5 | - |
Ectocarpus siliculosus |
| Mep7 | - |
Ectocarpus siliculosus |
| MEP7-C5 | - |
Ectocarpus siliculosus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | Ectocarpus contains a multigenic family of putative ManC5-Es. The genome sequence of Ectocarpus offers the opportunity to have access to a higher number of genes, and potentially proteins, 31 putative ManC5-E genes are analyzed. The ManC5-E family includes genes that are differentially regulated during the life cycle of Ectocarpus | Ectocarpus siliculosus |
| additional information | transcript expression as a function of the developmental program of the brown alga, Ectocarpus sp. | Ectocarpus siliculosus |
| physiological function | mannuronan C5-epimerases (ManC5-Es) catalyze in brown algae the remodeling of alginate, a major cell-wall component which is involved in many biological functions in these organisms. ManC5-Es are present as large multigenic families in brown algae, likely indicating functional specificities and specializations. ManC5-Es control the distribution pattern of (1-4)-linked beta-D-mannuronic acid (M) and alpha-L-guluronic acid (G) residues in alginates, giving rise to widely different polysaccharide compositions and sequences, depending on tissue, season, age, or algal species. Alginate in brown algae is first formed as a polysaccharide chain containing mannuronic acid residues only. These are subsequently transformed by the ManC5-E into guluronic acid residues, generating distinct patterns arranged in regions of MM-, GG- and MG-blocks. Patterns containing large stretches of adjacent guluronic acid residues (GG-blocks) form structured interchain associations in the presence of Ca2+ ions. These interchain junctions have the socalled egg-box conformation and are responsible for the gelling properties of alginate and cell-wall strengthening | Ectocarpus siliculosus |
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