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Literature summary for 5.1.3.31 extracted from
- Cloning, expression and purification of D-tagatose 3-epimerase gene from Escherichia coli JM109 (2015), Protein Expr. Purif., 114, 77-81 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | D-tagatose 3-epimerase is a useful enzyme for the production of expensive, rare sugars, e.g. D-sorbose and D-psicose, from inexpensive sugars, e.g. D-tagatose and D-fructose | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ycjR, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose | Escherichia coli | - |
D-psicose | - |
r |  |
| D-fructose | Escherichia coli JM109 | - |
D-psicose | - |
r | |
| D-tagatose | Escherichia coli | - |
D-sorbose | - |
r | |
| D-tagatose | Escherichia coli JM109 | - |
D-sorbose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | A0A037YIF5 | - |
- |
| Escherichia coli JM109 | A0A037YIF5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose | - |
Escherichia coli | D-psicose | - |
r | |
| D-fructose | - |
Escherichia coli JM109 | D-psicose | - |
r | |
| D-tagatose | - |
Escherichia coli | D-sorbose | - |
r | |
| D-tagatose | - |
Escherichia coli JM109 | D-sorbose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 29800, recombinant His-tagged enzyme, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DTE | - |
Escherichia coli |
| ycjR | - |
Escherichia coli |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | analysis of molecular clusters of DTE gene sequences, phylogenetic analysis, overview | Escherichia coli |
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