BRENDA - Enzyme Database show
show all sequences of 5.1.3.25

A new route to dTDP-6-deoxy-L-talose and dTDP-L-rhamnose: dTDP-L-rhamnose 4-epimerase in Burkholderia thailandensis

Yoo, H.G.; Kwon, S.Y.; Karki, S.; Kwon, H.J.; Bioorg. Med. Chem. Lett. 21, 3914-3917 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene BTH_I1476, DNA and amino acid sequence determination and analysis
Burkholderia thailandensis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0247
-
dTDP-6-deoxy-beta-L-talose
pH 8.2, temperature not specified in the publication
Burkholderia thailandensis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dTDP-6-deoxy-beta-L-talose
Burkholderia thailandensis
-
dTDP-beta-L-rhamnose
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Burkholderia thailandensis
Q2SYH7
gene BTH_I1476
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dTDP-6-deoxy-beta-L-talose
-
727136
Burkholderia thailandensis
dTDP-beta-L-rhamnose
-
-
-
r
dTDP-6-deoxy-beta-L-talose
the equilibrium of WibBBth catalysis favors dTDP-beta-L-rhamnose formation
727136
Burkholderia thailandensis
dTDP-beta-L-rhamnose
-
-
-
r
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.9
-
dTDP-6-deoxy-beta-L-talose
pH 8.2, temperature not specified in the publication
Burkholderia thailandensis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
8.4
in phosphate buffer
Burkholderia thailandensis
Cloned(Commentary) (protein specific)
Commentary
Organism
gene BTH_I1476, DNA and amino acid sequence determination and analysis
Burkholderia thailandensis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0247
-
dTDP-6-deoxy-beta-L-talose
pH 8.2, temperature not specified in the publication
Burkholderia thailandensis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dTDP-6-deoxy-beta-L-talose
Burkholderia thailandensis
-
dTDP-beta-L-rhamnose
-
-
r
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dTDP-6-deoxy-beta-L-talose
-
727136
Burkholderia thailandensis
dTDP-beta-L-rhamnose
-
-
-
r
dTDP-6-deoxy-beta-L-talose
the equilibrium of WibBBth catalysis favors dTDP-beta-L-rhamnose formation
727136
Burkholderia thailandensis
dTDP-beta-L-rhamnose
-
-
-
r
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.9
-
dTDP-6-deoxy-beta-L-talose
pH 8.2, temperature not specified in the publication
Burkholderia thailandensis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
8.4
in phosphate buffer
Burkholderia thailandensis
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
36.4
-
dTDP-6-deoxy-beta-L-talose
pH 8.2, temperature not specified in the publication
Burkholderia thailandensis
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
36.4
-
dTDP-6-deoxy-beta-L-talose
pH 8.2, temperature not specified in the publication
Burkholderia thailandensis
Other publictions for EC 5.1.3.25
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
727136
Yoo
A new route to dTDP-6-deoxy-L- ...
Burkholderia thailandensis
Bioorg. Med. Chem. Lett.
21
3914-3917
2011
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