Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activates | Acetoanaerobium sticklandii | |
additional information | pyridoxal 5'-phosphate is not involved in racemization | Acetoanaerobium sticklandii | |
SH compounds | activate, e.g. 2-mercaptoethanol, 1,3-dimercaptoethanol | Acetoanaerobium sticklandii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
lipoic acid | weak | Acetoanaerobium sticklandii | |
reduced glutathione | weak | Acetoanaerobium sticklandii | |
thioglycolate | weak | Acetoanaerobium sticklandii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 37 mM, 25% inhibition | Acetoanaerobium sticklandii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetoanaerobium sticklandii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Acetoanaerobium sticklandii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-proline = D-proline | racemization is accompanied by deuterium incorporation from the solvent into the alpha position of Pro, participation of two equivalent hydrogen acceptor sites | Acetoanaerobium sticklandii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.8 | - |
- |
Acetoanaerobium sticklandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Pro | - |
Acetoanaerobium sticklandii | D-Pro | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | 37 | at 37°C the activity is 35-40% greater than at 24°C | Acetoanaerobium sticklandii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.6 | 8.1 | pH 5.5: no detectable activity, rapid fall of activity below pH 6.5, activity is relatively stable between pH 6.7 and 8.1 | Acetoanaerobium sticklandii |