Literature summary for 4.99.1.2 extracted from

Nagata, T.; Muraoka, T.; Kiyonoa, M.; Pan-Hou, H.
Development of a luminescence-based biosensor for detection of methylmercury (2010), J. Toxicol. Sci., 35, 231-234.

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Application

Application	Comment	Organism
environmental protection	A recombinant whole-cell bacterial sensor for highly selective and sensitive detection of bioavailable methylmercury in the environment is constructed. The biosensor carries luciferase gene as a reporter under control of a very selective Hg2+-inducible part of the mer-operon from Pseudomonas K-62 plasmid pMR26. A merB gene encoding organomercurial lyase which cleaves the C-Hg bond of methylmercury to give Hg2+ is coexpressed in the sensor.	Pseudomonas sp. K-62

Cloned(Commentary)

Cloned (Comment)	Organism
co-expression in Escherichia coli DH5alpha with the luciferase gene luxAB from Vibrio harveyi	Pseudomonas sp. K-62

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp. K-62	O07303	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CH3Hg+ + H+	-	Pseudomonas sp. K-62	Hg2+ + CH4	-	?

Synonyms

Synonyms	Comment	Organism
merB	-	Pseudomonas sp. K-62
organomercurial lyase	-	Pseudomonas sp. K-62

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Release 2023.1 (January 2023)