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Literature summary for 4.4.1.4 extracted from

  • Kulikova, V.; Morozova, E.; Rodionov, A.; Koval, V.; Anufrieva, N.; Revtovich, S.; Demidkina, T.
    Non-stereoselective decomposition of (+-)-S-alk(en)yl-l-cysteine sulfoxides to antibacterial thiosulfinates catalyzed by C115H mutant methionine gamma-lyase from Citrobacter freundii (2018), Biochimie, 151, 42-44 .
    View publication on PubMed
Search for more literature for 4.4.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Citrobacter freundii

Protein Variants

Protein Variants Comment Organism
C115H mutant cleaves S-alk(en)yl-L-cysteine sulfoxides more effectively than the wild type Citrobacter freundii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.47
-
 (+-)-ethiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
0.54
-
 (+)-methiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
0.57
-
 (+-)-methiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
1.1
-
 (+-)-propiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
1.2
-
 (+)-alliin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
1.26
-
 (+-)-alliin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
1.4
-
 (+)-propiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii

Organism

Organism UniProt Comment Textmining
Citrobacter freundii Q84AR1 cf. EC 4.4.1.11
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-alliin + H2O
-
 Citrobacter freundii allicin + pyruvate + NH3
-
 ?
(+)-methiin + H2O
-
 Citrobacter freundii methanesulfenic acid + pyruvate + NH3
-
 ?
(+)-propiin + H2O
-
 Citrobacter freundii propane-1-sulfenic acid + pyruvate + NH3
-
 ?
(+-)-alliin + H2O
-
 Citrobacter freundii allicin + pyruvate + NH3
-
 ?
(+-)-ethiin + H2O
-
 Citrobacter freundii ethanesulfenic acid + pyruvate + NH3
-
 ?
(+-)-methiin + H2O
-
 Citrobacter freundii methanesulfenic acid + pyruvate + NH3
-
 ?
(+-)-propiin + H2O
-
 Citrobacter freundii propane-1-sulfenic acid + pyruvate + NH3
-
 ?
additional information enzyme additionally catalyzes the beta-elimination reaction of (+-)-S-alk(en)yl-L-cysteine sulfoxides to yield thiosulfinate, reaction of EC 4.4.1.4. It can decompose both diastereomers equally Citrobacter freundii ?
-
 ?
S-butyl-L-cysteine + H2O
-
 Citrobacter freundii butanethiol + NH3 + 2-oxopropanoate
-
 ?
S-ethyl-L-cysteine + H2O
-
 Citrobacter freundii ethanethiol + NH3 + 2-oxopropanoate
-
 ?
S-methyl-L-cysteine + H2O
-
 Citrobacter freundii methanethiol + NH3 + 2-oxopropanoate
-
 ?
S-propyl-L-cysteine + H2O
-
 Citrobacter freundii propanethiol + NH3 + 2-oxopropanoate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.14
-
 (+-)-methiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
5.2
-
 (+)-methiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
5.59
-
 (+-)-ethiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
12.4
-
 (+-)-alliin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
13.7
-
 (+)-alliin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
20.2
-
 (+-)-propiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
21.8
-
 (+)-propiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
9
-
 (+-)-methiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
9.6
-
 (+)-methiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
9.8
-
 (+-)-alliin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
11
-
 (+)-alliin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
12
-
 (+-)-ethiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
16
-
 (+)-propiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
18
-
 (+-)-propiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii