Protein Variants | Comment | Organism |
---|---|---|
C220A | largely catalytically inactive protein, 0.06% of wild-type activity | Xanthobacter autotrophicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | weak competitive | Xanthobacter autotrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | - |
epoxypropane | - |
Xanthobacter autotrophicus | |
0.034 | - |
CoM | - |
Xanthobacter autotrophicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | the inactive and Zn-deficient form of enzyme is activated by addition of ZnCl2 or CoCl2 | Xanthobacter autotrophicus | |
Zn | Zn plays a key role in activating an organic thiol substrate for nucleophilic attack on an alkyl-donating substrate, removal of Zn results in loss of catalytic activity, the inactive and Zn-deficient form of enzyme is activated by addition of ZnCl2 or CoCl2. Thermodynamic characterization of the interaction of CoM with an enzyme-bound Zn center | Xanthobacter autotrophicus | |
Zn2+ | the inactive and Zn-deficient form of enzyme is activated by addition of ZnCl2 or CoCl2 | Xanthobacter autotrophicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthobacter autotrophicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Xanthobacter autotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
epoxypropane + 3-mercaptopropionate | 0.59% of the activity with HS-CoM | Xanthobacter autotrophicus | 3-[(2-hydroxypropyl)thio]propanoic acid | - |
? | |
epoxypropane + HS-CoA | 0.51% of the activity with HS-CoM | Xanthobacter autotrophicus | ? | - |
? | |
epoxypropane + HS-CoA | 0.11% of the activity with HS-CoM | Xanthobacter autotrophicus | ? | - |
? | |
epoxypropane + HS-CoM | - |
Xanthobacter autotrophicus | 2-hydroxypropyl-CoM | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.5 | - |
epoxypropane | - |
Xanthobacter autotrophicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Xanthobacter autotrophicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.5 | pH 7.0: about 85% of maximal activity, pH 9.5: about 90% of maximal activity | Xanthobacter autotrophicus |