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Literature summary for 4.3.2.2 extracted from

  • Galina, L.; Dalberto, P.; Martinelli, L.; Roth, C.; Pinto, A.; Villela, A.; Bizarro, C.; MacHado, P.; Timmers, L.; De Souza, O.; De Carvalho Filho, E.; Basso, L.; Santos, D.
    Biochemical, thermodynamic and structural studies of recombinant homotetrameric adenylosuccinate lyase from Leishmania braziliensis (2017), RSC Adv., 7, 54347-54360 .
No PubMed abstract available
Search for more literature for 4.3.2.2

Application

Application Comment Organism
drug development as Leishmania species lack the de novo pathway and are dependent on the salvage pathway to supply their purine requirements, LbASL could thus represent a drug target for the development of chemical agents to treat leishmaniasis Leishmania braziliensis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Leishmania braziliensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0111
-
 (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate pH 7.5, 25°C Leishmania braziliensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate Leishmania braziliensis the enzyme is involved in both de novo and salvage pathways of purine biosynthesis fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
 ?
N6-(1,2-dicarboxyethyl)AMP Leishmania braziliensis the enzyme is involved in both de novo and salvage pathways of purine biosynthesis fumarate + AMP
-
 ?

Organism

Organism UniProt Comment Textmining
Leishmania braziliensis A4H3W5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Leishmania braziliensis

Storage Stability

Storage Stability Organism
-20°C or -80°C, pH 7.0, 150 mM KCl, 1 mM EDTA, 1 mM DTT and 10% glycerol, 3 months, the recombinant enzyme loses more than 50% of initial activity Leishmania braziliensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate
-
 Leishmania braziliensis fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
 ?
(S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate the enzyme is involved in both de novo and salvage pathways of purine biosynthesis Leishmania braziliensis fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
 ?
N6-(1,2-dicarboxyethyl)AMP the enzyme is involved in both de novo and salvage pathways of purine biosynthesis Leishmania braziliensis fumarate + AMP
-
 ?
N6-(1,2-dicarboxyethyl)AMP the enzyme follows a Uni-Bi ordered kinetic mechanism, in which release of fumarate is followed by AMP to yield free enzyme Leishmania braziliensis fumarate + AMP
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 51000, SDS-PAGE Leishmania braziliensis

Synonyms

Synonyms Comment Organism
ASL
-
 Leishmania braziliensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
337
-
 (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate pH 7.5, 25°C Leishmania braziliensis

General Information

General Information Comment Organism
metabolism the enzyme is involved in both de novo and salvage pathways of purine biosynthesis Leishmania braziliensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
30360
-
 (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate pH 7.5, 25°C Leishmania braziliensis