Literature summary for 4.3.2.1 extracted from

Tischner, R.; Galli, M.; Heimer, Y.M.; Bielefeld, S.; Okamoto, M.; Mack, A.; Crawford, N.M.
Interference with the citrulline-based nitric oxide synthase assay by argininosuccinate lyase activity in Arabidopsis extracts (2007), FEBS J., 274, 4238-4245.

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Inhibitors

Inhibitors	Comment	Organism	Structure
argininosuccinate	0.3 mM inhibits the reaction by 97%	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4.5	-	fumarate	-	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-(Nomega-L-arginino)succinate	Arabidopsis thaliana	-	fumarate + L-arginine	-	r

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
leaf extract is centrifuged, the supernatant lyophilized, the resuspended material is partially purified on a G-15 Sephadex and a Q-Sepharose FF column	Arabidopsis thaliana

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-(Nomega-L-arginino)succinate	-	Arabidopsis thaliana	fumarate + L-arginine	-	r
arginine + fumarate	-	Arabidopsis thaliana	argininosuccinate	-	r

Synonyms

Synonyms	Comment	Organism
Argininosuccinate lyase	-	Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	activity assay	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	activity assay	Arabidopsis thaliana

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Release 2023.1 (January 2023)