Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Cistanche deserticola |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-aminoindan-2-phosphonic acid | competitive, specific inhibitor | Cistanche deserticola | |
chlorogenic acid | uncompetitive | Cistanche deserticola | |
Cinnamic acid | mixed type | Cistanche deserticola | |
cinnamyl alcohol | uncompetitive | Cistanche deserticola | |
coniferyl alcohol | competitive | Cistanche deserticola | |
coniferyl aldehyde | competitive | Cistanche deserticola | |
coumaric acid | uncompetitive | Cistanche deserticola | |
Hg2+ | 1 mM, 65% loss of activity | Cistanche deserticola | |
additional information | not inhibitory: tyrosol, caffeic acid | Cistanche deserticola | |
Pb2+ | 1 mM, 50% loss of activity | Cistanche deserticola | |
salidroside | competitive | Cistanche deserticola | |
Zn2+ | 1 mM, 65% loss of activity | Cistanche deserticola |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.101 | - |
L-Phe | pH 8.5, 55°C | Cistanche deserticola |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cs+ | 1 mM, weak enhancement | Cistanche deserticola | |
Mn2+ | 1 mM, weak enhancement | Cistanche deserticola |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
94000 | - |
x * 94000, SDS-PAGE of recombinant protein with GST-tag | Cistanche deserticola |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cistanche deserticola | D3Y5L6 | isoform PAL1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Phe | - |
Cistanche deserticola | (E)-cinnamate + NH3 | - |
? | |
additional information | no substrate: L-Tyr | Cistanche deserticola | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 94000, SDS-PAGE of recombinant protein with GST-tag | Cistanche deserticola |
Synonyms | Comment | Organism |
---|---|---|
PAL1 | - |
Cistanche deserticola |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Cistanche deserticola |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
20 min, retains more than 85% of activity | Cistanche deserticola |
75 | - |
20 min, loss of more than 85% of activity | Cistanche deserticola |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.36 | - |
L-Phe | pH 8.5, 55°C | Cistanche deserticola |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Cistanche deserticola |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000056 | - |
2-aminoindan-2-phosphonic acid | pH 8.5, 55°C | Cistanche deserticola | |
0.008 | - |
Cinnamic acid | pH 8.5, 55°C | Cistanche deserticola | |
0.013 | - |
coniferyl aldehyde | pH 8.5, 55°C | Cistanche deserticola | |
0.017 | - |
salidroside | pH 8.5, 55°C | Cistanche deserticola | |
0.032 | - |
coniferyl alcohol | pH 8.5, 55°C | Cistanche deserticola | |
0.032 | - |
coumaric acid | pH 8.5, 55°C | Cistanche deserticola | |
0.037 | - |
cinnamyl alcohol | pH 8.5, 55°C | Cistanche deserticola | |
0.043 | - |
chlorogenic acid | pH 8.5, 55°C | Cistanche deserticola |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33.17 | - |
L-Phe | pH 8.5, 55°C | Cistanche deserticola |