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Literature summary for 4.3.1.24 extracted from
- Microbial L-phenylalanine ammonia-lyase. Purification, subunit structure and kinetic properties of the enzyme from Rhizoctonia solani (1975), Biochem. J., 149, 65-72.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | slight | Rhizoctonia solani |  |
| 4-coumarate | - |
Rhizoctonia solani | |
| caffeate | - |
Rhizoctonia solani | |
| CN- | irreversible; NaCN | Rhizoctonia solani | |
| D-Phe | - |
Rhizoctonia solani | |
| dihydrocaffeic acid | - |
Rhizoctonia solani | |
| dithiothreitol | slight | Rhizoctonia solani | |
| GSH | slight | Rhizoctonia solani | |
| L-Cys | slight | Rhizoctonia solani | |
| NaBH4 | irreversible | Rhizoctonia solani | |
| NaHSO3 | irreversible | Rhizoctonia solani | |
| p-chloromercuribenzoate | slight | Rhizoctonia solani | |
| phenylpyruvate | - |
Rhizoctonia solani | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
alpha2,beta2, 2 * 70000 + 2 * 90000, SDS-PAGE | Rhizoctonia solani |
| 90000 | - |
alpha2,beta2, 2 * 70000 + 2 * 90000, SDS-PAGE | Rhizoctonia solani |
| 330000 | - |
gel filtration | Rhizoctonia solani |
| 340000 | - |
density gradient centrifugation | Rhizoctonia solani |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizoctonia solani | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhizoctonia solani |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rhizoctonia solani |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,4-dihydroxyphenylalanine | 29% of the activity with L-Phe | Rhizoctonia solani | ? | - |
? | |
| fluorophenylalanine | p-fluorophenylalanine, 31% of the activity with L-Phe, o-fluorophenylalanine, 14% of the activity with L-Phe | Rhizoctonia solani | trans-(4-fluoro)cinnamate + NH3 | - |
? | |
| L-Phe | - |
Rhizoctonia solani | (E)-cinnamate + NH3 | - |
? | |
| L-tyrosine | m-Tyr, 10% of the activity with L-Phe | Rhizoctonia solani | p-coumarate + NH3 | - |
? | |
| L-tyrosine | L-Tyr, 15% of the activity with L-Phe | Rhizoctonia solani | p-coumarate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | alpha2,beta2, 2 * 70000 + 2 * 90000, SDS-PAGE | Rhizoctonia solani |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 44 | 46 | - |
Rhizoctonia solani |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.7 | 8.9 | - |
Rhizoctonia solani |
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Release 2023.1 (January 2023)
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