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Literature summary for 4.3.1.2 extracted from

  • Kato, Y.; Asano, Y.
    Purification and properties of crystalline 3-methylaspartase from two facultative anaerobes, Citrobacter sp. strain YG-0504 and Morganella morganii strain YG-0601 (1995), Biosci. Biotechnol. Biochem., 59, 93-99.
    View publication on PubMed
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Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Citrobacter freundii
-
 Morganella morganii

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate) 1 mM, complete Citrobacter freundii
5,5'-dithiobis(2-nitrobenzoate) 1 mM 54%, loss of activity Morganella morganii
8-hydroxyquinoline 5 mM, 44% loss of activity Citrobacter freundii
8-hydroxyquinoline 5 mM, 88% loss of activity Morganella morganii
Ag+ complete Citrobacter freundii
Ag+ complete Morganella morganii
Al3+ 5 mM AlCl3, 95% loss of activity Morganella morganii
Ca2+ CaCl2 5 mM, 83% loss of activity Citrobacter freundii
Ca2+
-
 Clostridium tetanomorphum
Ca2+ 5 mM CaCl2, 73% loss of activity Morganella morganii
Cd2+ 1 mM CdCl2, complete inhibition Morganella morganii
Co2+ 1 mM CoCl2, 60% loss of activity Morganella morganii
Cu2+ 1 mM CuSO4, 47% loss of activity Citrobacter freundii
Cu2+
-
 Clostridium tetanomorphum
Cu2+ 1 mM CuSO4, 73% loss of activity Morganella morganii
Fe2+ 5 mM FeSO4, 56% loss of activity Citrobacter freundii
Fe2+ 5 mM FeSO4, 89% loss of activity Morganella morganii
Fe3+ 1 mM, 75% loss of activity Citrobacter freundii
Fe3+ 1 mM, 69% loss of activity Morganella morganii
Hg+ complete Citrobacter freundii
Hg+ complete Morganella morganii
Hg2+ complete Citrobacter freundii
Hg2+ complete Morganella morganii
iodoacetate 1 mM, 86% loss of activity Morganella morganii
additional information
-
 Citrobacter freundii
additional information
-
 Morganella morganii
N-ethylmaleimide complete Citrobacter freundii
N-ethylmaleimide
-
 Clostridium tetanomorphum
N-ethylmaleimide complete Morganella morganii
p-chloromercuribenzoate complete Citrobacter freundii
p-chloromercuribenzoate complete Morganella morganii
Pb2+ 5 mM PbCl2, 88% loss of activity Morganella morganii
Sn2+ 1 mM SnCl2, 51% loss of activity Morganella morganii

Metals/Ions

Metals/Ions Comment Organism Structure
Cs+
-
 Citrobacter freundii
Cs+
-
 Morganella morganii
Li+
-
 Citrobacter freundii
Li+
-
 Morganella morganii
Mg2+
-
 Citrobacter freundii
Mg2+
-
 Clostridium tetanomorphum
Mg2+
-
 Morganella morganii
Mn2+ activates Citrobacter freundii
Mn2+ activates Clostridium tetanomorphum
Mn2+ activates Morganella morganii
Na+
-
 Citrobacter freundii
Na+
-
 Morganella morganii
Ni2+ activates Citrobacter freundii
Ni2+ activates Clostridium tetanomorphum
Ni2+ activates Morganella morganii
Rb+
-
 Citrobacter freundii
Rb+
-
 Morganella morganii
Zn2+ activates Citrobacter freundii
Zn2+ activates Clostridium tetanomorphum
Zn2+ activates Morganella morganii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 2 * 40000, SDS-PAGE Citrobacter freundii
44000
-
 2 * 44000, SDS-PAGE Morganella morganii
70000
-
 gel filtration Morganella morganii
79000
-
 gel filtration Citrobacter freundii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-threo-3-methylaspartate Citrobacter freundii
-
 ?
-
 ?
L-threo-3-methylaspartate Clostridium tetanomorphum
-
 ?
-
 ?
L-threo-3-methylaspartate Morganella morganii
-
 ?
-
 ?
additional information Clostridium tetanomorphum enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes ?
-
 ?
additional information Clostridium tetanomorphum NCIMB 11547 enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes ?
-
 ?

Organism

Organism UniProt Comment Textmining
Citrobacter freundii
-
 Citrobacter sp. strain YG-0504
-
Clostridium tetanomorphum
-
 NCIMB 11547
-
Clostridium tetanomorphum NCIMB 11547
-
 NCIMB 11547
-
Morganella morganii
-
-
-
Morganella morganii YG-0601
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Citrobacter freundii
-
 Morganella morganii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Citrobacter freundii
additional information
-
-
 Clostridium tetanomorphum
additional information
-
-
 Morganella morganii
162
-
-
 Citrobacter freundii
296
-
-
 Morganella morganii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S,3S)-3-ethylaspartic acid
-
 Citrobacter freundii ?
-
 ?
(2S,3S)-3-ethylaspartic acid
-
 Clostridium tetanomorphum ?
-
 ?
(2S,3S)-3-ethylaspartic acid
-
 Morganella morganii ?
-
 ?
3-chloroaspartate
-
 Clostridium tetanomorphum chlorofumarate + NH3
-
 ?
3-chloroaspartate (2R,3S)-3-chloroaspartic acid Citrobacter freundii chlorofumarate + NH3
-
 ?
3-chloroaspartate (2R,3S)-3-chloroaspartic acid Morganella morganii chlorofumarate + NH3
-
 ?
3-chloroaspartate
-
 Clostridium tetanomorphum NCIMB 11547 chlorofumarate + NH3
-
 ?
3-chloroaspartate (2R,3S)-3-chloroaspartic acid Morganella morganii YG-0601 chlorofumarate + NH3
-
 ?
? + NH4Cl
-
 Citrobacter freundii ?
-
 ?
? + NH4Cl
-
 Morganella morganii ?
-
 ?
ethylfumarate + NH3
-
 Citrobacter freundii ?
-
 ?
ethylfumarate + NH3
-
 Clostridium tetanomorphum ?
-
 ?
ethylfumarate + NH3
-
 Morganella morganii ?
-
 ?
ethylfumarate + NH3
-
 Morganella morganii YG-0601 ?
-
 ?
L-threo-3-methylaspartate r Citrobacter freundii mesaconate + NH3
-
 ?
L-threo-3-methylaspartate r Clostridium tetanomorphum mesaconate + NH3
-
 ?
L-threo-3-methylaspartate r Morganella morganii mesaconate + NH3
-
 ?
L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Citrobacter freundii mesaconate + NH3
-
 ?
L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Clostridium tetanomorphum mesaconate + NH3
-
 ?
L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Morganella morganii mesaconate + NH3
-
 ?
L-threo-3-methylaspartate r Clostridium tetanomorphum NCIMB 11547 mesaconate + NH3
-
 ?
L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Clostridium tetanomorphum NCIMB 11547 mesaconate + NH3
-
 ?
L-threo-3-methylaspartate r Morganella morganii YG-0601 mesaconate + NH3
-
 ?
L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Morganella morganii YG-0601 mesaconate + NH3
-
 ?
L-threo-3-methylaspartate
-
 Citrobacter freundii ?
-
 ?
L-threo-3-methylaspartate
-
 Clostridium tetanomorphum ?
-
 ?
L-threo-3-methylaspartate
-
 Morganella morganii ?
-
 ?
additional information
-
 Citrobacter freundii ?
-
 ?
additional information
-
 Clostridium tetanomorphum ?
-
 ?
additional information
-
 Morganella morganii ?
-
 ?
additional information enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes Clostridium tetanomorphum ?
-
 ?
additional information
-
 Clostridium tetanomorphum NCIMB 11547 ?
-
 ?
additional information enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes Clostridium tetanomorphum NCIMB 11547 ?
-
 ?
additional information
-
 Morganella morganii YG-0601 ?
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Clostridium tetanomorphum
dimer 2 * 44000, SDS-PAGE Morganella morganii
dimer 2 * 40000, SDS-PAGE Citrobacter freundii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 maximal activity in the deamination reaction of L-threo-3-methylaspartate Morganella morganii
50
-
 maximal activity in the deamination reaction of L-threo-3-methylaspartate Citrobacter freundii
55
-
-
 Clostridium tetanomorphum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
-
 Citrobacter freundii
additional information
-
-
 Morganella morganii
45
-
 pH 7.0, 10 min, 0.1 M potassium phosphate buffer, deamination reaction of (2S,3S)-3-methylaspartate, 5% loss of activity Citrobacter freundii
45
-
 pH 7.0, 10 min, 0.1 M potassium phosphate buffer, deamination reaction of (2S,3S)-3-methylaspartate, 5% loss of activity Morganella morganii
50
-
 0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 50% loss of activity Citrobacter freundii
50
-
 0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 50% loss of activity Morganella morganii
55
-
 0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, most of activity is lost Citrobacter freundii
55
-
 0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, most of activity is lost Morganella morganii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 maximal activity in TRIS/HCl buffer in the amination reaction of mesaconate Morganella morganii
8.5
-
 maximal activity in TRIS/HCl buffer in the amination reaction of mesaconate Citrobacter freundii
9.7
-
 maximal activity in ethanolamine/HCl buffer in the deamination reaction of L-threo-3-methylaspartate Citrobacter freundii
9.7
-
 maximal activity in ethanolamine/HCl buffer in the deamination reaction of L-threo-3-methylaspartate Morganella morganii

pH Range

pH Minimum pH Maximum Comment Organism
6 11 pH 6.0: 10% of maximal activity, pH 11.0: 40% of maximal activity in the amination reaction of mesaconate Morganella morganii
7 11 pH 7.0: about 10% of maximal activity, pH 11.0: about 80% of maximal activity in the deamination reaction of L-threo-3-methylaspartate Morganella morganii
7 11 pH 7.0: about 25% of maximal activity, pH 11.0: about 5% of maximal activity, in the deamination reaction of L-threo-3-methylaspartate Citrobacter freundii
7 11 pH 7.0: about 10% of maximal activity, pH 11.0: about 90% of maximal activity in the amination reaction of mesaconate Citrobacter freundii

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
-
 Citrobacter freundii
additional information
-
-
 Morganella morganii
5 10 stable between, pH 5.0: inactivated rapidly below Citrobacter freundii
6 8.5 stable between, pH 5.0: inactivated rapidly below Morganella morganii