Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
Crystallization (Comment) | Organism |
---|---|
both wild-type and selenomethionine labelled holoenzyme, to 2.4 and 1.9 A resolution, respectively. Significant electron density is not observed for the cofactor pyridoxal 5'-phosphate. Wild-type enzyme is in an open conformation while selenomethionine enzyme, crystallized in the presence of isoserine, is in a closed conformation suggesting that the enzyme is likely to undergo conformational changes upon binding of substrate. Thr166 may be involved in abstraction of proton from the Calpha atom of the substrate | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
D-Ser | pH 7.5, 37°C, buffer K+-phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.45 | - |
D-Thr | pH 7.5, 37°C, buffer Na+-phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.53 | - |
D-Thr | pH 7.5, 37°C, buffer K+-phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.63 | - |
D-allo-Thr | pH 7.5, 37°C, buffer Na+-phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.87 | - |
D-Ser | pH 7.5, 37°C, buffer Na+-phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.88 | - |
D-allo-Thr | pH 7.5, 37°C, buffer K+-phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | |
10.2 | - |
L-Ser | pH 7.5, 37°C, buffer K+-phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | |
11.8 | - |
L-Ser | pH 7.5, 37°C, buffer Na+-phosphate | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.09 | - |
wild-type, pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium |
2.12 | - |
selenomethionine labelled enzyme, pH 7.5, 37°C | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-allo-Thr | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 2-oxobutanoate + NH3 | - |
? | |
D-Ser | - |
Salmonella enterica subsp. enterica serovar Typhimurium | pyruvate + NH3 | - |
? | |
D-Thr | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 2-oxobutanoate + NH3 | - |
? | |
L-Ser | - |
Salmonella enterica subsp. enterica serovar Typhimurium | pyruvate + NH3 | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
DSD | - |
Salmonella enterica subsp. enterica serovar Typhimurium |