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Literature summary for 4.3.1.10 extracted from

  • Murakoshi, I.; Sanda, A.; Haginiwa, J.
    L-Serine O-sulphate lyase, a new enzyme in extracts from higher plants (1977), Chem. Pharm. Bull., 25, 1829-1832.
No PubMed abstract available
Search for more literature for 4.3.1.10

Inhibitors

Inhibitors Comment Organism Structure
cyanide 1.25 mM, complete inhibition Leucaena leucocephala
D-phospho-L-serine weak competitive Leucaena leucocephala
D-serine O-sulfate
-
 Leucaena leucocephala
hydrazine 25 mM, complete inhibition Leucaena leucocephala
hydroxylamine 25 mM, complete inhibition Leucaena leucocephala
O-phospho-L-serine weak non-competitive Leucaena leucocephala
pyridoxal 5'-phosphate 0.06 mg/l, 25-30% inhibition Leucaena leucocephala

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine O-sulfate Vigna radiata var. radiata biosynthesis of cysteine derivates ?
-
 ?
L-serine O-sulfate Pisum sativum biosynthesis of cysteine derivates ?
-
 ?
L-serine O-sulfate Raphanus sativus biosynthesis of cysteine derivates ?
-
 ?
L-serine O-sulfate Allium cepa biosynthesis of cysteine derivates ?
-
 ?
L-serine O-sulfate Brassica rapa subsp. oleifera biosynthesis of cysteine derivates ?
-
 ?
L-serine O-sulfate Citrullus lanatus subsp. vulgaris biosynthesis of cysteine derivates ?
-
 ?
L-serine O-sulfate Allium sativum biosynthesis of cysteine derivates ?
-
 ?
L-serine O-sulfate Mimosa pudica biosynthesis of cysteine derivates ?
-
 ?
L-serine O-sulfate Leucaena leucocephala biosynthesis of cysteine derivates ?
-
 ?
L-serine O-sulfate Fagopyrum esculentum biosynthesis of cysteine derivates ?
-
 ?

Organism

Organism UniProt Comment Textmining
Allium cepa
-
-
-
Allium sativum
-
-
-
Brassica rapa subsp. oleifera
-
-
-
Citrullus lanatus subsp. vulgaris
-
-
-
Fagopyrum esculentum
-
-
-
Leucaena leucocephala
-
-
-
Mimosa pudica
-
-
-
Pisum sativum
-
-
-
Raphanus sativus
-
-
-
Vigna radiata var. radiata
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
bulb
-
 Allium cepa
-
bulb
-
 Allium sativum
-
seedling
-
 Vigna radiata var. radiata
-
seedling
-
 Pisum sativum
-
seedling
-
 Raphanus sativus
-
seedling
-
 Brassica rapa subsp. oleifera
-
seedling
-
 Citrullus lanatus subsp. vulgaris
-
seedling
-
 Mimosa pudica
-
seedling
-
 Leucaena leucocephala
-
seedling
-
 Fagopyrum esculentum
-

Storage Stability

Storage Stability Organism
0°C, for 25 h, remaining activity of 84% Leucaena leucocephala

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine O-sulfate optimum concentration 188 mM Leucaena leucocephala pyruvate + NH3 + sulfate
-
 ?
L-serine O-sulfate quite specific Vigna radiata var. radiata pyruvate + NH3 + sulfate
-
 ?
L-serine O-sulfate quite specific Pisum sativum pyruvate + NH3 + sulfate
-
 ?
L-serine O-sulfate quite specific Raphanus sativus pyruvate + NH3 + sulfate
-
 ?
L-serine O-sulfate quite specific Allium cepa pyruvate + NH3 + sulfate
-
 ?
L-serine O-sulfate quite specific Brassica rapa subsp. oleifera pyruvate + NH3 + sulfate
-
 ?
L-serine O-sulfate quite specific Citrullus lanatus subsp. vulgaris pyruvate + NH3 + sulfate
-
 ?
L-serine O-sulfate quite specific Allium sativum pyruvate + NH3 + sulfate
-
 ?
L-serine O-sulfate quite specific Mimosa pudica pyruvate + NH3 + sulfate
-
 ?
L-serine O-sulfate quite specific Leucaena leucocephala pyruvate + NH3 + sulfate
-
 ?
L-serine O-sulfate quite specific Fagopyrum esculentum pyruvate + NH3 + sulfate
-
 ?
L-serine O-sulfate biosynthesis of cysteine derivates Vigna radiata var. radiata ?
-
 ?
L-serine O-sulfate biosynthesis of cysteine derivates Pisum sativum ?
-
 ?
L-serine O-sulfate biosynthesis of cysteine derivates Raphanus sativus ?
-
 ?
L-serine O-sulfate biosynthesis of cysteine derivates Allium cepa ?
-
 ?
L-serine O-sulfate biosynthesis of cysteine derivates Brassica rapa subsp. oleifera ?
-
 ?
L-serine O-sulfate biosynthesis of cysteine derivates Citrullus lanatus subsp. vulgaris ?
-
 ?
L-serine O-sulfate biosynthesis of cysteine derivates Allium sativum ?
-
 ?
L-serine O-sulfate biosynthesis of cysteine derivates Mimosa pudica ?
-
 ?
L-serine O-sulfate biosynthesis of cysteine derivates Leucaena leucocephala ?
-
 ?
L-serine O-sulfate biosynthesis of cysteine derivates Fagopyrum esculentum ?
-
 ?
L-threonine O-sulfate activity: 1/30 that towards L-serine O-sulfate Leucaena leucocephala 2-oxobutanoate + NH3 + SO42-
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.9
-
-
 Leucaena leucocephala

pH Range

pH Minimum pH Maximum Comment Organism
8.5 10 about 65% of the maximal activity at pH 8.5 and about 35% of maximal activity at pH 10.0 in Tris-HCl buffer Leucaena leucocephala