Literature summary for 4.2.99.B1 extracted from

Longley, M.J.; Prasad, R.; Srivastava, D.K.; Wilson, S.H.; Copeland, W.C.
identification of 5'-deoxyribose phosphate lyase activity in human DNA polymerase gamma and its role in mitochondrial base excision repair in vitro (1998), Proc. Natl. Acad. Sci. USA, 95, 12244-12248.

Search for more literature for 4.2.99.B1

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0006	-	preincised apurinic/apyrimidinic DNA	pH 7.4, 37°C, in presence of 0.2 mM EDTA	Homo sapiens
0.007	-	preincised apurinic/apyrimidinic DNA	recombinant protein, pH 7.4, 37°C, in presence of 0.2 mM EDTA	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Homo sapiens	5739	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	divalent metal ions are noch required	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
catalysis of the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site in DNA where a DNA-(apurinic or apyrimidinic site) lyase has already cleaved the C-O-P bond 3' to the apurinic or apyrimidinic site	the catalytic subunit of pol gamma catalyzes the release of the the 5'-deoxyribose phosphate residue from incised apurinic/apyrimidinic sites to produce a substrate for DNA ligase. The ability to trap covalent enzyme DNA complexes with NaBH4 implicates Schiff base intermediate in an beta-elimination reaction mechanism	Homo sapiens	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
49-bp oligodeoxynucleotide containing uracil at position 21	labelled at 3'-end with [alpha-32P]ddATP by terminal deoxynucleotidyl transferase and annealed to an unlabelled complementary strand containing a G residue opposite the uracil. Before use, the substrate is treated with uracil-DNA glycosylase and AP endonuclease to generate a single nucleotide gap directly upstream from the labelled fragment containing a deoxyribose phosphate flap	Homo sapiens	? + 2-deoxy-D-ribose 5-phosphate	-	?
preincised apurinic/apyrimidinic DNA	-	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
More	dRP lyase activity is intrinsic to the catalytic subunit of human pol gamma	Homo sapiens

Synonyms

Synonyms	Comment	Organism
5'-deoxyribose phosphate lyase	-	Homo sapiens
DNA polymerase gamma	with 5'-deoxyribose phosphate lyase activity	Homo sapiens
Pol gamma	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0043	-	preincised apurinic/apyrimidinic DNA	pH 7.4, 37°C, in presence of 0.2 mM EDTA	Homo sapiens
4.5	-	preincised apurinic/apyrimidinic DNA	recombinant protein, pH 7.4, 37°C, in presence of 0.2 mM EDTA	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)