Cloned (Comment) | Organism |
---|---|
recombinant pol lambda and the K310A mutant are overexpressed in Eschercihia coli and purified to near homogeneity | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K310A | eliminates more than 90% of the wild-type dRP lyase activity, indicating that Lys 310 is the main nucleophile involved in the reaction, forming the Schiff base internediate during beta-elimination | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9UGP5 | the 8-kDa-domain of pol lambda, residues 240-325 show high amino acid similarity (30%) with that of pol beta, catalyzing the same reaction, residues 1-87, UniProt-ID: P06746 | - |
Purification (Comment) | Organism |
---|---|
recombinant pol lambda and the K310A mutant are overexpressed in Eschercihia coli and purified to near homogeneity | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
catalysis of the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site in DNA where a DNA-(apurinic or apyrimidinic site) lyase has already cleaved the C-O-P bond 3' to the apurinic or apyrimidinic site | homologous to the dRP lyase activity of polymerase beta, pol beta. Proposed to participate in single-nucleotide base excision repair in mammalian cells, which is the major repair pathway in eukaryotic cells responsible for repair of lesions that give rise to abasic siles in DNA | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
34-bp oligonucleotide containing uracil at position 16 | labelled with 3'-end by terminal deoxynucleotidyltransferase using [alpha-32P]ddATP and annealed to its complementary oligonucleotide, and pretreated with human uracil-DNA glycosylase and AP endonuclease | Homo sapiens | ? + 2-deoxy-D-ribose 5-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5'-deoxyribose-5-phosphate lyase | - |
Homo sapiens |
DNA polymerase lambda | dRP lyase activity | Homo sapiens |
dRP lyase | - |
Homo sapiens |
Pol lambda | - |
Homo sapiens |