Literature summary for 4.2.99.B1 extracted from

Lopes, D.d.O.; Schamber-Reis, B.L.F.; Regis-da-Silva, C.G.; Rajao, M.A.; DaRocha, W.D.; Macedo, A.M.; Franco, G.R.; Nardelli, S.C.; Schenkman, S.; Hoffmann, J.-S.; Cazaux, C.; Pena, S.D.J.; Teixeira, S.M.R.; Machado, C.R.
Biochemical studies with DNA polymerase beta and DNA polymerase beta -PAK of Trypanosoma cruzi suggest the involvement of these proteins in mitochondrial DNA maintenance (2008), DNA Repair, 7, 1882-1892.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain BL21 (DE3) competent cells	Trypanosoma cruzi

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma cruzi	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	Trypanosoma cruzi

Reaction

Reaction	Comment	Organism	Reaction ID
catalysis of the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site in DNA where a DNA-(apurinic or apyrimidinic site) lyase has already cleaved the C-O-P bond 3' to the apurinic or apyrimidinic site	TcPolbeta and TcPolbetaPAK, homologous to mammalian DNA polymerasebeta both show dRP lyase activity. The efficiency is not as high as the human Polbeta enzyme	Trypanosoma cruzi	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
34-mer-deoxyoligonucleotide containing a uracil residue at position 16	pretreated with uracil-N-glycosylase and apurunic/apyrimidinic endonuclease	Trypanosoma cruzi	18-mer-deoxyoligonucleotide + 2-deoxy-D-ribose 5-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
dRP lyase	-	Trypanosoma cruzi
TcPolbeta	-	Trypanosoma cruzi
TcPolbetaPAK	-	Trypanosoma cruzi

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Trypanosoma cruzi

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Trypanosoma cruzi

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Release 2023.1 (January 2023)