Crystallization (Comment) | Organism |
---|---|
hanging drop method, crystallographic analyses of wild-type enzyme and a promiscuous mutant, with unreactive 2-fluoro analogues ((2E,6E)-2-fluorofarnesyl diphosphate and (2Z,6E)-2-fluorofarnesyl diphosphate) of (2E,6E)- and (2Z,6E)-farnesyl diphosphate reveals catalytically relevant binding modes and distinct farnesyl chain topologies that are consistent with preorganization by the active-site for cisoid or transoid cyclization | Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00797 | - |
(2Z,6E)-farnesyl diphosphate | pH 7.5, 22°C, promiscuous mutant enzyme M4 | Nicotiana tabacum | |
0.01403 | - |
(2Z,6E)-farnesyl diphosphate | pH 7.5, 22°C, wild-type enzyme | Nicotiana tabacum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2Z,6E)-farnesyl diphosphate | Nicotiana tabacum | - |
(-)-beta-curcumene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2Z,6E)-farnesyl diphosphate | - |
Nicotiana tabacum | (-)-beta-curcumene + diphosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.077 | - |
(2Z,6E)-farnesyl diphosphate | pH 7.5, 22°C, promiscuous mutant enzyme M4 | Nicotiana tabacum | |
0.095 | - |
(2Z,6E)-farnesyl diphosphate | pH 7.5, 22°C, wild-type enzyme | Nicotiana tabacum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.77 | - |
(2Z,6E)-farnesyl diphosphate | pH 7.5, 22°C, wild-type enzyme | Nicotiana tabacum | |
9.66 | - |
(2Z,6E)-farnesyl diphosphate | pH 7.5, 22°C, promiscuous mutant enzyme M4 | Nicotiana tabacum |