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Literature summary for 4.2.3.93 extracted from

  • Kumeta, Y.; Ito, M.
    Characterization of alpha-humulene synthases responsible for the production of sesquiterpenes induced by methyl jasmonate in Aquilaria cell culture (2016), J. Nat. Med., 70, 452-459 .
    View publication on PubMed
Search for more literature for 4.2.3.93

Cloned(Commentary)

Cloned (Comment) Organism
gene AcC2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, heterologous expression of the enzyme in Escherichia coli Aquilaria crassna

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information the enzyme has no N-terminal transit peptide Aquilaria crassna
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate Aquilaria crassna
-
 alpha-guaiene + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate Aquilaria crassna
-
 delta-guaiene + diphosphate
-
 ?
additional information Aquilaria crassna the enzyme catalyzes the production of three sesquiterpenes, alpha-guaiene, alpha-humulene, and delta-guaiene ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aquilaria crassna D0VMR6
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information the enzyme has no N-terminal transit peptide Aquilaria crassna

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Aquilaria crassna
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
 Aquilaria crassna alpha-guaiene + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate
-
 Aquilaria crassna delta-guaiene + diphosphate
-
 ?
additional information the enzyme catalyzes the production of three sesquiterpenes, alpha-guaiene, alpha-humulene, and delta-guaiene Aquilaria crassna ?
-
 ?
additional information solid-phase microextraction gas chromatography/mass spectrometry (SPME-GC/MS) analysis of the enzyme reaction products Aquilaria crassna ?
-
 ?

Synonyms

Synonyms Comment Organism
ACC2
-
 Aquilaria crassna
delta-guaiene synthase 1
-
 Aquilaria crassna

Expression

Organism Comment Expression
Aquilaria crassna methyl jasmonate induces the expression of enzyme delta-guaiene synthase, at 6 h after methyl jasmonate treatment, the expression of the delta-guaiene synthase gene has increased approximately 150fold resulting in 240 pmol/mg protein/h delta-guaiene production up

General Information

General Information Comment Organism
metabolism the enzyme is involved in the biosynthetic pathways for sesquiterpenes found in agarwood and cell suspension cultures, putative pathway, overview. Various post-transcriptional, translational, or post-translational regulatory mechanisms affecting the expression or activity of alpha-humulene and delta-guaiene synthases are possible Aquilaria crassna
additional information the enzyme contains conserved RPx8W motif and DDxxD motif, which is a divalent metal-ion substrate-binding site Aquilaria crassna
physiological function the enzyme is involved in the biosynthesis of the three sesquiterpenes, alpha-guaiene (cf. EC 4.2.3.87), alpha-humulene (cf. EC 4.2.3.104), and delta-guaiene, only very small amounts of alpha-humulene are produced Aquilaria crassna