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Literature summary for 4.2.3.82 extracted from

  • Hua, G.; Hu, Y.; Yang, C.; Liu, D.; Mao, Z.; Zhang, L.; Zhang, Y.
    Characterization of santalene synthases using an inorganic pyrophosphatase coupled colorimetric assay (2018), Anal. Biochem., 547, 26-36 .
    View publication on PubMed
Search for more literature for 4.2.3.82

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Santalum album
DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Santalum spicatum
DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Clausena lansium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetic analysis, overview. Kinetics are measured by IPP1 coupled colorimetric assay Santalum album
additional information
-
 additional information Michaelis-Menten kinetic analysis, overview. Kinetics are measured by IPP1 coupled colorimetric assay Santalum spicatum
additional information
-
 additional information Michaelis-Menten kinetic analysis, overview. Kinetics are measured by IPP1 coupled colorimetric assay Clausena lansium
0.0015
-
 (2E,6E)-farnesyl diphosphate recombinant His-tagged enzyme, pH 7.4, 37°C Clausena lansium
0.002
-
 (2E,6E)-farnesyl diphosphate recombinant His-tagged enzyme, pH 7.4, 37°C Santalum spicatum
0.0027
-
 (2E,6E)-farnesyl diphosphate recombinant His-tagged enzyme, pH 7.4, 37°C Santalum album

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Santalum album
Mg2+ required for activity Santalum spicatum
Mg2+ required for activity Clausena lansium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate Santalum album
-
 (+)-alpha-santalene + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate Santalum spicatum
-
 (+)-alpha-santalene + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate Clausena lansium
-
 (+)-alpha-santalene + diphosphate
-
 ?
additional information Santalum spicatum the enzyme produces (+)-alpha-santalene, (-)-beta-santalene, and (-)-exo-alpha-bergamotene, and traces of (-)-epi-beta-santalene from (2E,6E)-farnesyl diphosphate ?
-
 ?
additional information Clausena lansium the enzyme produces high amonuts of (+)-alpha-santalene from (2E,6E)-farnesyl diphosphate, and traces of (-)-exo-alpha-bergamotene ?
-
 ?
additional information Santalum album the enzyme produces high amounts of (+)-alpha-santalene and (-)-beta-santalene, also (-)-exo-alpha-bergamotene, and low amounts of (-)-epi-beta-santalene from (2E,6E)-farnesyl diphosphate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Clausena lansium E5LLI1
-
-
Santalum album E3W202
-
-
Santalum spicatum E3W204
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by streptomycin sulfate and ammonium sulfate fractionation, desalting gel filtration, and anion exchange chromatography, followed by another step of ammonium sulfate fractionation and desalting gel filtration to near homogeneity Clausena lansium
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) ickel affinity chromatography, dialysis, and ultrafiltration to near homogeneity Santalum album
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) ickel affinity chromatography, dialysis, anion exchange chromatography, and gel filtration to near homogeneity Santalum spicatum

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Clausena lansium
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.067
-
 purified recombinant His-tagged enzyme, pH 7.4, 37°C Santalum album
0.069
-
 purified recombinant enzyme, substrate (2E,6E)-farnesyl diphosphate, pH 7.4, 37°C Santalum spicatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
 Santalum album (+)-alpha-santalene + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate
-
 Santalum spicatum (+)-alpha-santalene + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate
-
 Clausena lansium (+)-alpha-santalene + diphosphate
-
 ?
additional information the enzyme produces (+)-alpha-santalene, (-)-beta-santalene, and (-)-exo-alpha-bergamotene, and traces of (-)-epi-beta-santalene from (2E,6E)-farnesyl diphosphate Santalum spicatum ?
-
 ?
additional information the enzyme produces high amonuts of (+)-alpha-santalene from (2E,6E)-farnesyl diphosphate, and traces of (-)-exo-alpha-bergamotene Clausena lansium ?
-
 ?
additional information the enzyme produces high amounts of (+)-alpha-santalene and (-)-beta-santalene, also (-)-exo-alpha-bergamotene, and low amounts of (-)-epi-beta-santalene from (2E,6E)-farnesyl diphosphate Santalum album ?
-
 ?
additional information the enzyme produces high amonuts of (+)-alpha-santalene and (-)-exo-alpha-bergamotene, also (-)-beta-santalene, and traces of (-)-epi-beta-santalene from (2E,6E)-farnesyl diphosphate. The enzyme assay is coupled to the inorganic diphosphatase (IPP1) from Saccharomyces cerevisiae. Compound identification and quantification by GC-MS and NMR analysis Santalum spicatum ?
-
 ?
additional information the enzyme produces high amonuts of alpha-santalene from (2E,6E)-farnesyl diphosphate, and traces of (-)-exo-alpha-bergamotene. The enzyme assay is coupled to the inorganic diphosphatase (IPP1) from Saccharomyces cerevisiae. Compound identification and quantification by GC-MS and NMR analysis Clausena lansium ?
-
 ?
additional information the enzyme produces high amounts of (+)-alpha-santalene and (-)-beta-santalene, also (-)-exo-alpha-bergamotene, and low amounts of (-)-epi-beta-santalene from (2E,6E)-farnesyl diphosphate. The enzyme assay is coupled to the inorganic diphosphatase (IPP1) from Saccharomyces cerevisiae. Compound identification and quantification by GC-MS and NMR analysis Santalum album ?
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Santalum spicatum

Synonyms

Synonyms Comment Organism
SanSyn
-
 Clausena lansium
santalene synthase
-
 Santalum album
santalene synthase
-
 Santalum spicatum
santalene synthase
-
 Clausena lansium
SaSSy
-
 Santalum album
SspiSy
-
 Santalum spicatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Santalum album
37
-
 assay at Santalum spicatum
37
-
 assay at Clausena lansium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.073
-
 (2E,6E)-farnesyl diphosphate recombinant His-tagged enzyme, pH 7.4, 37°C Santalum album
0.075
-
 (2E,6E)-farnesyl diphosphate recombinant His-tagged enzyme, pH 7.4, 37°C Clausena lansium
0.107
-
 (2E,6E)-farnesyl diphosphate recombinant His-tagged enzyme, pH 7.4, 37°C Santalum spicatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Santalum album
7.4
-
 assay at Santalum spicatum
7.4
-
 assay at Clausena lansium

General Information

General Information Comment Organism
physiological function the enzyme is involved in sandalwood oil biosynthesis Santalum album
physiological function the enzyme is involved in sandalwood oil biosynthesis Santalum spicatum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
27.04
-
 (2E,6E)-farnesyl diphosphate recombinant His-tagged enzyme, pH 7.4, 37°C Santalum album
50
-
 (2E,6E)-farnesyl diphosphate recombinant His-tagged enzyme, pH 7.4, 37°C Clausena lansium
53.3
-
 (2E,6E)-farnesyl diphosphate recombinant His-tagged enzyme, pH 7.4, 37°C Santalum spicatum