Literature summary for 4.2.3.74 extracted from

Pinedo, C.; Wang, C.; Pradier, J.; Dalmais, B.; Choquer, M.; Le, P.P.; Morgant, G.; Collado, I.G.; Cane, D.E.; Viaud, M.
Sesquiterpene synthase from the botrydial biosynthetic gene cluster of the phytopathogen Botrytis cinerea (2008), ACS Chem. Biol., 3, 791-801.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Botrytis cinerea

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00604	-	(2E,6E)-farnesyl diphosphate	recombinant enzyme, in 50 mM PIPES, 10 mM MgCl2, 100 mM NaCl, pH 7.0, at 30°C	Botrytis cinerea

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	the BOT2 protein has both of the two strictly conserved active-site motifs that are essential to binding of three Mg2+ cations	Botrytis cinerea

Organism

Organism	UniProt	Comment	Textmining
Botrytis cinerea	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography	Botrytis cinerea

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate + H2O	-	Botrytis cinerea	presilphiperfolan-8beta-ol + diphosphate	-	?
additional information	the BOT2 protein is a sesquiterpene synthase	Botrytis cinerea	?	-	?

Synonyms

Synonyms	Comment	Organism
BOT2	-	Botrytis cinerea

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.23	-	(2E,6E)-farnesyl diphosphate	recombinant enzyme, in 50 mM PIPES, 10 mM MgCl2, 100 mM NaCl, pH 7.0, at 30°C	Botrytis cinerea

General Information

General Information	Comment	Organism
malfunction	Botrytis cinerea BOT2 deletion mutants are blocked in the first step of botrydial biosynthesis	Botrytis cinerea
metabolism	BOT2 is required for the first step of botrydial biosynthesis	Botrytis cinerea

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Release 2023.1 (January 2023)