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Literature summary for 4.2.3.61 extracted from

  • Brodelius, M.; Lundgren, A.; Mercke, P.; Brodelius, P.
    Fusion of farnesyldiphosphate synthase and epi-aristolochene synthase, a sesquiterpene cyclase involved in capsidiol biosynthesis in Nicotiana tabacum (2002), Eur. J. Biochem., 269, 3570-3577.
    View publication on PubMed
Search for more literature for 4.2.3.61

Application

Application Comment Organism
synthesis construction of fusion proteins with farnesyl diphosphate synthase FPPS from Artemisia annua. The fusion enzymes produce epi-aristolochene from isopentenyl diphosphate through a coupled reaction. The Km values of FPPS and eAS for isopentenyl diphosphate and farnesyl diphosphate, respectively, are essentially the same for the single and fused enzymes. The bifunctional enzymes show a more efficient conversion of isopentenyl diphosphate to epi-aristolochene than the corresponding amount of single enzymes Nicotiana tabacum

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Nicotiana tabacum

Protein Variants

Protein Variants Comment Organism
additional information construction of fusion proteins with farnesyl diphosphate synthase FPPS from Artemisia annua. The fusion enzymes produce epi-aristolochene from isopentenyl diphosphate through a coupled reaction. The Km values of FPPS and eAS for isopentenyl diphosphate and farnesyl diphosphate, respectively, are essentially the same for the single and fused enzymes. The bifunctional enzymes show a more efficient conversion of isopentenyl diphosphate to epi-aristolochene than the corresponding amount of single enzymes Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
 (2E,6E)-farnesyl diphosphate fusion construct epiaristolochene synthase/farnesyl diphosphate synthase, pH 8.0, 30°C Nicotiana tabacum
0.0017
-
 (2E,6E)-farnesyl diphosphate wild-type, pH 8.0, 30°C Nicotiana tabacum
0.0026
-
 (2E,6E)-farnesyl diphosphate fusion construct farnesyl diphosphate synthase/epiaristolochene synthase, pH 8.0, 30°C Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
 Nicotiana tabacum (+)-5-epiaristolochene + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
EAS
-
 Nicotiana tabacum