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Literature summary for 4.2.3.61 extracted from

  • Faraldos, J.A.; OMaille, P.E.; Dellas, N.; Noel, J.P.; Coates, R.M.
    Bisabolyl-derived sesquiterpenes from tobacco 5-epi-aristolochene synthase-catalyzed cyclization of (2Z,6E)-farnesyl diphosphate (2010), J. Am. Chem. Soc., 132, 4281-4289.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0084
-
 (2E,6E)-farnesyl diphosphate pH 7.0, 22°C Nicotiana tabacum
0.014
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 22°C Nicotiana tabacum
0.0197
-
 (2E,6Z)-6-fluorofarnesyl diphosphate pH 7.0, 22°C Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
 Nicotiana tabacum (+)-5-epiaristolochene + diphosphate
-
 ?
(2E,6Z)-6-fluorofarnesyl diphosphate the fluoro substitution at the C6 position of farnesyl diphosphate has negligible effects on enzyme binding, substrate orientation, diphosphate ionization, and the initial 1,10 ring closure catalyzed by TEAS Nicotiana tabacum (-)-1-fluorogermacrene A sole product, 58% yield ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.042
-
 (2E,6E)-farnesyl diphosphate pH 7.0, 22°C Nicotiana tabacum
0.095
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 22°C Nicotiana tabacum
0.11
-
 (2E,6Z)-6-fluorofarnesyl diphosphate pH 7.0, 22°C Nicotiana tabacum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5
-
 (2E,6E)-farnesyl diphosphate pH 7.0, 22°C Nicotiana tabacum
5.6
-
 (2E,6Z)-6-fluorofarnesyl diphosphate pH 7.0, 22°C Nicotiana tabacum
6.7
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 22°C Nicotiana tabacum