Literature summary for 4.2.3.6 extracted from

Vedula, L.S.; Zhao, Y.; Coates, R.M.; Koyama, T.; Cane, D.E.; Christianson, D.W.
Exploring biosynthetic diversity with trichodiene synthase (2007), Arch. Biochem. Biophys., 466, 260-266.

Search for more literature for 4.2.3.6

Cloned(Commentary)

Cloned (Comment)	Organism
for expression in Escherichia coli BL21DE3 cells	Fusarium sporotrichioides

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystallized by the hanging drop vapor diffusion method, in complex with Mg2+, PPi, the benzyl triethylammonium cation, at a resolution of 2.85 A	Fusarium sporotrichioides

Inhibitors

Inhibitors	Comment	Organism	Structure
benzyl triethylammonium cation	BTAC alone does not inhibit the trichodiene synthase, 5-30 microM acts as a competitive inhibitor in the presence of 5-20 microM PPi	Fusarium sporotrichioides
diphosphate	PPi	Fusarium sporotrichioides

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Fusarium sporotrichioides

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
89000	-	-	Fusarium sporotrichioides

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
trans,trans-farnesyl diphosphate	Fusarium sporotrichioides	-	trichodiene + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Fusarium sporotrichioides	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Fusarium sporotrichioides

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-fluorofarnesyl diphosphate	-	Fusarium sporotrichioides	?	sesquiterpene mixture	?
4-methylfarnesyl diphosphate	-	Fusarium sporotrichioides	?	sesquiterpene mixture	?
trans,trans-farnesyl diphosphate	-	Fusarium sporotrichioides	trichodiene + diphosphate	-	?

Subunits

Subunits	Comment	Organism
homodimer	-	Fusarium sporotrichioides

Synonyms

Synonyms	Comment	Organism
trichodiene synthase	-	Fusarium sporotrichioides

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	activity assay	Fusarium sporotrichioides

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.8	-	activity assay	Fusarium sporotrichioides

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00049	-	diphosphate	-	Fusarium sporotrichioides
0.0007	-	diphosphate	apparent inhibition constant of PPi in the prensence of BTAC	Fusarium sporotrichioides
0.036	-	benzyl triethylammonium cation	induced inhibition constant for BTAC in the presence of PPi	Fusarium sporotrichioides

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Release 2023.1 (January 2023)