Cloned (Comment) | Organism |
---|---|
gene TPS1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, the enzyme encoded by gene TPS1 produces beta-caryophyllene as a main product and humulene as a minor compound, and thus is named caryophyllene synthase (PnCPS), illumina transcriptome sequencing of unripe peppercorn identifiying sesquiterpene synthases (sesqui-TPSs), recombinant expression of His-tagged enzyme TPS1 in Escherichia coli strain Rosetta 2(DE3) | Piper nigrum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten steady-state kinetics | Piper nigrum | |
0.0211 | - |
(2E,6E)-farnesyl diphosphate | pH not specified in the publication, 30°C, recombinant His-tagged enzyme | Piper nigrum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | Piper nigrum | the enzyme stereochemistry is not determined in the publication | beta-caryophyllene + diphosphate | - |
? | |
additional information | Piper nigrum | the enzyme encoded by gene TPS1 produces beta-caryophyllene as a main product and humulene as a minor compound, and thus is named caryophyllene synthase (PnCPS) | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Piper nigrum | A0A1V0E492 | from garden of Prince of Songkla University, Thailand | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme TPS1 from Escherichia coli strain Rosetta 2(DE3) by nickel affinity chromatography, desalting gel filtration, and ultrafiltration | Piper nigrum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(2E,6E)-farnesyl diphosphate = (-)-beta-caryophyllene + diphosphate | possible mechanism of sesquiterpene synthases from Piper nigrum, the reaction mechanism includes a 1,10-ring closure, overview | Piper nigrum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | unripe peppercorn is subjected to the Illumina transcriptome sequencing, young, unripe, and ripe fruits. GC/MS profiling of terpenes from immature black pepper fruit, accumulation of beta-caryophyllene, the major sesquiterpene in peppercorn, changes from 46% to 70% as fruits ripen | Piper nigrum | - |
leaf | low level | Piper nigrum | - |
stem | high level | Piper nigrum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | the enzyme stereochemistry is not determined in the publication | Piper nigrum | beta-caryophyllene + diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate | the enzyme stereochemistry is not determined in the publication, the reaction mechanism includes a 1,10-ring closure | Piper nigrum | beta-caryophyllene + diphosphate | - |
? | |
additional information | the enzyme encoded by gene TPS1 produces beta-caryophyllene as a main product and humulene as a minor compound, and thus is named caryophyllene synthase (PnCPS) | Piper nigrum | ? | - |
? | |
additional information | steady-state kinetic parameters are determined using malachite green phosphate assay | Piper nigrum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 64900, about, sequence calculation | Piper nigrum |
Synonyms | Comment | Organism |
---|---|---|
More | cf. EC 4.2.3.89 | Piper nigrum |
PnCPS | - |
Piper nigrum |
PnTPS1 | - |
Piper nigrum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Piper nigrum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.455 | - |
(2E,6E)-farnesyl diphosphate | pH not specified in the publication, 30°C, recombinant His-tagged enzyme | Piper nigrum |
General Information | Comment | Organism |
---|---|---|
evolution | the cyclization process catalyzed by PnTPSs follows a 1,10-closure mechanism assuming that the present PnTPSs have evolved from a common ancestor | Piper nigrum |
metabolism | GC/MS profiling of terpenes from immature black pepper fruit, accumulation of beta-caryophyllene, the major sesquiterpene in peppercorn, changes from 46% to 70% as fruits ripen | Piper nigrum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21.6 | - |
(2E,6E)-farnesyl diphosphate | pH not specified in the publication, 30°C, recombinant His-tagged enzyme | Piper nigrum |