Application | Comment | Organism |
---|---|---|
drug development | enzymes of shikimate pathway are attractive targets to the development of antitubercular agents | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
cloning and expression in Escherichia coli strain Rosetta(DE3) in the absence of IPTG induction, heterologous overexpression of the MtCS protein | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41800 | - |
determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis and electrospray mass spectrometry | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-O-(1-carboxyvinyl)-3-phosphoshikimate | Mycobacterium tuberculosis | seventh enzyme of the shikimate pathway, catalyzes the NADH- and FMN-dependent synthesis of chorismate, a precursor of aromatic amino acids, naphthoquinones, menaquinones, and mycobactins | chorismate + phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by using a 3-step purification (anion exchange, hydrophobic interaction and anion exchange chromatography) | Mycobacterium tuberculosis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Mycobacterium tuberculosis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
bifunctionality is determined by measurements of both chorismate synthase and NADH:FMN oxidoreductase activities | Mycobacterium tuberculosis |
additional information | - |
in the presence of oxygen the CS activity is limited by the reoxidation of FMNred cofactor | Mycobacterium tuberculosis |
0.004 | - |
CS activity | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-O-(1-carboxyvinyl)-3-phosphoshikimate | seventh enzyme of the shikimate pathway, catalyzes the NADH- and FMN-dependent synthesis of chorismate, a precursor of aromatic amino acids, naphthoquinones, menaquinones, and mycobactins | Mycobacterium tuberculosis | chorismate + phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | in solution, determined by gel filtration chromatography | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
5-O-(1-carboxyvinyl)-3-phosphoshikimate phosphate lyase | - |
Mycobacterium tuberculosis |
aroF | - |
Mycobacterium tuberculosis |
chorismate synthase | - |
Mycobacterium tuberculosis |
CS | - |
Mycobacterium tuberculosis |
MtCS | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at, CS activity and FMN-reductase activity | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0083 | - |
NADH | kinetics of reduction of the MtCS-bound FMNox is determined using fixed amount of holoenzyme and varying levels of NADH | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | reduced form | Mycobacterium tuberculosis |