Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | treatment of the Co2+-holoenzyme with EDTA in the absence of substrate results in the rapid loss of catalytic activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00055 | - |
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate | at 20°C and pH 8.5 | Escherichia coli | |
0.0006 | - |
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate | at 20°C and pH 6.5 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | reconstitution of the apoenzyme with Cd2+ restores activity to12% of the level observed with the Co2+-holoenzyme | Escherichia coli | |
Co2+ | contains 1 mol of Co2+, required for 100% activity | Escherichia coli | |
Cu2+ | reconstitution of the apoenzyme with Zn2+ restores activity to 9% of the level observed with the Co2+-holoenzyme | Escherichia coli | |
Mn2+ | reconstitution of the apoenzyme with Mn2+ restores activity to 10% of the level observed with the Co2+-holoenzyme | Escherichia coli | |
additional information | no activity in the presence of Mg2+, Ca2+, Sr2+, and Ba2+ | Escherichia coli | |
Ni2+ | reconstitution of the apoenzyme with Ni2+ restores activity to 23% of the level observed with the Co2+-holoenzyme | Escherichia coli | |
Zn2+ | reconstitution of the apoenzyme with Zn2+ restores activity to 53% of the level observed with the Co2+-holoenzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli RB791 | - |
- |
- |
Storage Stability | Organism |
---|---|
4°C, free enzyme in the presence of Co2+ and NAD+, at pH 7.5 in 100 mM MOPS buffer, several months, the enzyme remains stable | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate | - |
Escherichia coli | 3-dehydroquinate + phosphate | - |
? | |
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate | - |
Escherichia coli RB791 | 3-dehydroquinate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dehydroquinate synthase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
25000 | - |
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate | at 20°C and pH 7.8 | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | required for activity | Escherichia coli |