Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermus sp. GH5 |
Protein Variants | Comment | Organism |
---|---|---|
D100N | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Thermus sp. GH5 |
D100V | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Thermus sp. GH5 |
R80D/D100R | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Thermus sp. GH5 |
R80I | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Thermus sp. GH5 |
R80K | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Thermus sp. GH5 |
R80Q | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Thermus sp. GH5 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | allosteric inhibitor | Thermus sp. GH5 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
glycerone phosphate | mutant enzyme D100V, at pH 6.2 and 60°C | Thermus sp. GH5 | |
0.5 | - |
glycerone phosphate | mutant enzyme R80D/D100R, at pH 6.2 and 60°C | Thermus sp. GH5 | |
0.8 | - |
glycerone phosphate | mutant enzyme R8Q, at pH 6.2 and 60°C | Thermus sp. GH5 | |
0.8 | - |
glycerone phosphate | wild type enzyme, at pH 6.2 and 60°C | Thermus sp. GH5 | |
0.9 | - |
glycerone phosphate | mutant enzyme R80K, at pH 6.2 and 60°C | Thermus sp. GH5 | |
1 | - |
glycerone phosphate | mutant enzyme D100N, at pH 6.2 and 60°C | Thermus sp. GH5 | |
1.2 | - |
glycerone phosphate | mutant enzyme R80I, at pH 6.2 and 60°C | Thermus sp. GH5 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerone phosphate | Thermus sp. GH5 | - |
2-oxopropanal + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus sp. GH5 | B3VH91 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus sp. GH5 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerone phosphate | - |
Thermus sp. GH5 | 2-oxopropanal + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | - |
Thermus sp. GH5 |
Synonyms | Comment | Organism |
---|---|---|
MGS | - |
Thermus sp. GH5 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 80 | the enzyme shows no loss of activity after 150 min at 70°C, shows about 70% activity after 150 min at 75°C, and is almost inactivated after 60 min at 80°C | Thermus sp. GH5 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8 | - |
glycerone phosphate | mutant enzyme R8Q, at pH 6.2 and 60°C | Thermus sp. GH5 | |
13 | - |
glycerone phosphate | mutant enzyme D100V, at pH 6.2 and 60°C | Thermus sp. GH5 | |
15 | - |
glycerone phosphate | mutant enzyme R80I, at pH 6.2 and 60°C | Thermus sp. GH5 | |
29 | - |
glycerone phosphate | mutant enzyme D100N, at pH 6.2 and 60°C | Thermus sp. GH5 | |
38 | - |
glycerone phosphate | mutant enzyme R80K, at pH 6.2 and 60°C | Thermus sp. GH5 | |
162 | - |
glycerone phosphate | mutant enzyme R80D/D100R, at pH 6.2 and 60°C | Thermus sp. GH5 | |
218 | - |
glycerone phosphate | wild type enzyme, at pH 6.2 and 60°C | Thermus sp. GH5 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.8 | - |
glycerone phosphate | mutant enzyme R8Q, at pH 6.2 and 60°C | Thermus sp. GH5 | |
12.5 | - |
glycerone phosphate | mutant enzyme R80I, at pH 6.2 and 60°C | Thermus sp. GH5 | |
30 | - |
glycerone phosphate | mutant enzyme D100N, at pH 6.2 and 60°C | Thermus sp. GH5 | |
42 | - |
glycerone phosphate | mutant enzyme R80K, at pH 6.2 and 60°C | Thermus sp. GH5 | |
43 | - |
glycerone phosphate | mutant enzyme D100V, at pH 6.2 and 60°C | Thermus sp. GH5 | |
272 | - |
glycerone phosphate | wild type enzyme, at pH 6.2 and 60°C | Thermus sp. GH5 | |
324 | - |
glycerone phosphate | mutant enzyme R80D/D100R, at pH 6.2 and 60°C | Thermus sp. GH5 |