Protein Variants | Comment | Organism |
---|---|---|
additional information | a mutant carrying one additional His residue between residues Arg22 and His23 shos remarkable increase in thermostability, whereas a mutant carrying two additional His residues between residues Arg22 and His23 is very unstable. Replacing His23 in the latter mutant by Ala leads to a decrease in half-lifecompared to wild-type, but increase compared to the original mutant | Thermus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.67 | - |
dihydroxyacetone phosphate | carrying one additional His residue between residues Arg22 and His23, Hill coefficient 1.58, pH 6.2, 60°C | Thermus sp. | |
0.83 | - |
dihydroxyacetone phosphate | wild-type, Hill coefficient 2.2, pH 6.2, 60°C | Thermus sp. | |
1.45 | - |
dihydroxyacetone phosphate | carrying two additional His residues between residues Arg22 and His23, Hill coefficient 2.12, pH 6.2, 60°C | Thermus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus sp. | B3VH91 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroxyacetone phosphate | - |
Thermus sp. | methylglyoxal + phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
138 | - |
dihydroxyacetone phosphate | carrying two additional His residues between residues Arg22 and His23, Hill coefficient 2.12, pH 6.2, 60°C | Thermus sp. | |
153 | - |
dihydroxyacetone phosphate | carrying one additional His residue between residues Arg22 and His23, Hill coefficient 1.58, pH 6.2, 60°C | Thermus sp. | |
230 | - |
dihydroxyacetone phosphate | wild-type, Hill coefficient 2.2, pH 6.2, 60°C | Thermus sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
95 | - |
dihydroxyacetone phosphate | carrying two additional His residues between residues Arg22 and His23, Hill coefficient 2.12, pH 6.2, 60°C | Thermus sp. | |
227 | - |
dihydroxyacetone phosphate | carrying one additional His residue between residues Arg22 and His23, Hill coefficient 1.58, pH 6.2, 60°C | Thermus sp. | |
275 | - |
dihydroxyacetone phosphate | wild-type, Hill coefficient 2.2, pH 6.2, 60°C | Thermus sp. |