Literature summary for 4.2.3.27 extracted from

Yeom, S.J.; Kim, M.; Kim, S.K.; Lee, D.H.; Kwon, K.K.; Lee, H.; Kim, H.; Kim, D.M.; Lee, S.G.
Molecular and biochemical characterization of a novel isoprene synthase from Metrosideros polymorpha (2018), BMC Plant Biol., 18, 118 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Metrosideros polymorpha

Inhibitors

Inhibitors	Comment	Organism	Structure
Ca2+	about 50% residual activity at 1 mM	Metrosideros polymorpha
Co2+	about 75% residual activity at 1 mM	Metrosideros polymorpha
Cu2+	about 85% residual activity at 1 mM	Metrosideros polymorpha
EDTA	complete inhibition at 1 mM	Metrosideros polymorpha
Fe2+	about 5% residual activity at 1 mM	Metrosideros polymorpha
Mg2+	about 55% residual activity at 1 mM	Metrosideros polymorpha
Ni2+	about 80% residual activity at 1 mM	Metrosideros polymorpha
Zn2+	about 90% residual activity at 1 mM	Metrosideros polymorpha

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
8.11	-	dimethylallyl diphosphate	at pH 6.0 and 50°C	Metrosideros polymorpha

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	required, maximum activity at 5 mM Mn2+	Metrosideros polymorpha

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
65000	-	gel filtration	Metrosideros polymorpha

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
dimethylallyl diphosphate	Metrosideros polymorpha	-	isoprene + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Metrosideros polymorpha	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
IMAC column chromatography	Metrosideros polymorpha

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dimethylallyl diphosphate	-	Metrosideros polymorpha	isoprene + diphosphate	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 65000, SDS-PAGE	Metrosideros polymorpha
monomer	1 * 64950, calculated from amino acid sequence	Metrosideros polymorpha

Synonyms

Synonyms	Comment	Organism
ISPS	-	Metrosideros polymorpha

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
55	-	-	Metrosideros polymorpha

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
30	45	about 60% activity at 30°C, about 70% activity at 35°C, about 80% activity at 40°C, about 90% activity at 45°C and 55°C, about 85% activity at 60°C	Metrosideros polymorpha

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.35	-	dimethylallyl diphosphate	at pH 6.0 and 50°C	Metrosideros polymorpha

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Metrosideros polymorpha

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.5	7	at pH 5.5 and 7.0, the activity is approximately 40% of the maximum	Metrosideros polymorpha

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.043	-	dimethylallyl diphosphate	at pH 6.0 and 50°C	Metrosideros polymorpha

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Release 2023.1 (January 2023)