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Literature summary for 4.2.3.24 extracted from

  • Picaud, S.; Olofsson, L.; Brodelius, M.; Brodelius, P.E.
    Expression, purification, and characterization of recombinant amorpha-4,11-diene synthase from Artemisia annua L (2005), Arch. Biochem. Biophys., 436, 215-226.
    View publication on PubMed
Search for more literature for 4.2.3.24

Cloned(Commentary)

Cloned (Comment) Organism
-
 Artemisia annua
expression in Escherichia coli with His-tag Artemisia annua

Inhibitors

Inhibitors Comment Organism Structure
Co2+ inhibitory above 0.5 mM Artemisia annua
Cu2+ complete inhibition Artemisia annua
Mn2+ inhibitory above 0.5 mM Artemisia annua
Ni2+
-
 Artemisia annua
Zn2+ complete inhibition Artemisia annua

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006
-
 farnesyl diphosphate presence of Co2+, pH 7.5, 30°C Artemisia annua
0.0007
-
 farnesyl diphosphate presence of Co2+, pH 6.5, 30°C Artemisia annua
0.0016
-
 farnesyl diphosphate presence of Mg2+, pH 9.5, 30°C Artemisia annua
0.002
-
 farnesyl diphosphate presence of Mg2+, pH 7.5, 30°C Artemisia annua
0.0033
-
 farnesyl diphosphate presence of Mg2+, pH 6.5, 30°C Artemisia annua
0.0042
-
 farnesyl diphosphate presence of Mn2+, pH 7.5, 30°C Artemisia annua
0.008
-
 farnesyl diphosphate presence of Mn2+, pH 6.5, 30°C Artemisia annua
0.0169
-
 geranyl diphosphate presence of Mn2+, pH 6.5, 30°C Artemisia annua
0.0282
-
 geranyl diphosphate presence of Mn2+, pH 7.5, 30°C Artemisia annua

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ increase in product selectivity at pH 6.5, inhibitory above 0.5 mM Artemisia annua
Mg2+
-
 Artemisia annua
Mn2+ increase in product selectivity at pH 6.5, inhibitory above 0.5 mM Artemisia annua

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 x * 65000, SDS-PAGE, recombinant enzyme with His-tag Artemisia annua

Organism

Organism UniProt Comment Textmining
Artemisia annua
-
-
-
Artemisia annua
-
 expression in Escherichia coli with His-tag
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme with his-tag Artemisia annua

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
 Artemisia annua amorpha-4,11-diene + diphosphate dditionally production of 15 different sesquiterpenoids, detailed reaction scheme ?
farnesyl diphosphate
-
 Artemisia annua amorpha-4,11-diene + diphosphate additionally production of 15 different sesquiterpenoids, detailed reaction scheme ?
Geranyl diphosphate
-
 Artemisia annua ?
-
 ?
geranyl diphosphate alpha-terpineol (39%) + ocimene (24%) + myrcene (19%) + linalool (11%) Artemisia annua alpha-terpineol + ocimene + myrcene + linalool
-
 ?
geranyl diphosphate + H2O substrate in the presence of Mn2+ but not with Mg2+ or Co2+ Artemisia annua amorpha-4,11-diene + diphosphate
-
 ?
additional information significant increased product selectivity in the presence of Mn2+ or Co2+ Artemisia annua ?
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE, recombinant enzyme with His-tag Artemisia annua

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00034
-
 geranyl diphosphate presence of Mn2+, pH 7.5, 30°C Artemisia annua
0.0007
-
 geranyl diphosphate presence of Mn2+, pH 6.5, 30°C Artemisia annua
0.00078
-
 (2E,6E)-farnesyl diphosphate presence of Co2+, pH 7.5, 30°C Artemisia annua
0.0013
-
 (2E,6E)-farnesyl diphosphate presence of Co2+, pH 6.5, 30°C Artemisia annua
0.0043
-
 (2E,6E)-farnesyl diphosphate presence of Mg2+, pH 7.5, 30°C Artemisia annua
0.0068
-
 (2E,6E)-farnesyl diphosphate presence of Mg2+, pH 6.5, 30°C Artemisia annua
0.0108
-
 (2E,6E)-farnesyl diphosphate presence of Mn2+, pH 7.5, 30°C Artemisia annua
0.015
-
 (2E,6E)-farnesyl diphosphate presence of Mn2+, pH 6.5, 30°C Artemisia annua
0.0154
-
 (2E,6E)-farnesyl diphosphate presence of Mg2+, pH 9.5, 30°C Artemisia annua

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Artemisia annua

pH Range

pH Minimum pH Maximum Comment Organism
7.5
-
 pH-minimum, increasing enzyme activity with increasing pH above 7.5 Artemisia annua

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.012
-
 geranyl diphosphate presence of Mn2+, pH 7.5, 30°C Artemisia annua
0.042
-
 geranyl diphosphate presence of Mn2+, pH 6.5, 30°C Artemisia annua
1.3
-
 (2E,6E)-farnesyl diphosphate presence of Co2+, pH 7.5, 30°C Artemisia annua
1.9
-
 (2E,6E)-farnesyl diphosphate presence of Mn2+, pH 6.5, 30°C Artemisia annua
2.1
-
 (2E,6E)-farnesyl diphosphate presence of Co2+, pH 6.5, 30°C Artemisia annua
2.1
-
 (2E,6E)-farnesyl diphosphate presence of Mg2+, pH 6.5, 30°C Artemisia annua
2.2
-
 (2E,6E)-farnesyl diphosphate presence of Mg2+, pH 7.5, 30°C Artemisia annua
2.6
-
 (2E,6E)-farnesyl diphosphate presence of Mn2+, pH 7.5, 30°C Artemisia annua
9.7
-
 (2E,6E)-farnesyl diphosphate presence of Mg2+, pH 9.5, 30°C Artemisia annua