BRENDA - Enzyme Database
show all sequences of 4.2.3.136

Theoretical and experimental analysis of the reaction mechanism of MrTPS2, a triquinane-forming sesquiterpene synthase from chamomile

Hong, Y.J.; Irmisch, S.; Wang, S.C.; Garms, S.; Gershenzon, J.; Zu, L.; Koellner, T.G.; Tantillo, D.J.; Chemistry 19, 13590-13600 (2013)

Data extracted from this reference:

Engineering
Protein Variants
Commentary
Organism
Y512F
mutant produces (E)-beta-caryophyllene and alpha-humulene, not isocomene
Matricaria chamomilla
Organism
Organism
UniProt
Commentary
Textmining
Matricaria chamomilla
I6R4V5
var. recutita
-
Reaction
Reaction
Commentary
Organism
Reaction ID
(2E,6E)-farnesyl diphosphate = (-)-alpha-isocomene + diphosphate
reaction proceeds through caryophyllyl and presilphiperfolanyl cations and involves the protonation of a stable (-)-(E)-beta-caryophyllene intermediate
Matricaria chamomilla
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(2E,6E)-farnesyl diphosphate
reaction mechanism most likely involves repeated cycles of protonation/deprotonation of a neutral (-)-(E)-beta-caryophyllene intermediate. The deprotonation of the final isocomyl carbocation can occur either at C1 or C13, resulting in the main product alpha-isocomene and the minor product beta-isocomene
729600
Matricaria chamomilla
(-)-alpha-isocomene + diphosphate
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
TPS2
-
Matricaria chamomilla
Engineering (protein specific)
Protein Variants
Commentary
Organism
Y512F
mutant produces (E)-beta-caryophyllene and alpha-humulene, not isocomene
Matricaria chamomilla
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(2E,6E)-farnesyl diphosphate
reaction mechanism most likely involves repeated cycles of protonation/deprotonation of a neutral (-)-(E)-beta-caryophyllene intermediate. The deprotonation of the final isocomyl carbocation can occur either at C1 or C13, resulting in the main product alpha-isocomene and the minor product beta-isocomene
729600
Matricaria chamomilla
(-)-alpha-isocomene + diphosphate
-
-
-
?
Other publictions for EC 4.2.3.136
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
729600
Hong
Theoretical and experimental a ...
Matricaria chamomilla
Chemistry
19
13590-13600
2013
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719187
Irmisch
The organ-specific expression ...
Matricaria chamomilla var. recutita
BMC Plant Biol.
12
84
2012
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