Literature summary for 4.2.3.131 extracted from

Dai, Z.; Liu, Y.; Huang, L.; Zhang, X.
Production of miltiradiene by metabolically engineered Saccharomyces cerevisiae (2012), Biotechnol. Bioeng., 109, 2845-2853.

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Application

Application	Comment	Organism
synthesis	expression of copalyl diphosphate synthase and miltiradiene synthase in Saccharomyces cerevisiae, results in production of 4.2 mg/l miltiradiene. Miltiradiene production increases to 8.8 mg/l by improving supply of geranylgeranyl diphosphate by over-expression of a fusion gene of farnesyl diphosphate synthase ERG20 and endogenous geranylgeranyl diphosphate synthase BTS1 together with a heterologous geranylgeranyl diphosphate synthase from Sulfolobus acidocaldarius. Combinatorial overexpression of truncated 3-hydroxyl-3-methylglutaryl-CoA reductase with a mutated global regulatory factor upc2.1 and ERG20-BTS1-geranylgeranyl diphosphate synthase genes has synergetic effects on miltiradiene production, increasing titer to 61.8 mg/l. In fed-batch fermentation 488 mg/l miltiradiene can be produced	Salvia miltiorrhiza

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli and Saccharomyces cerevisiae	Salvia miltiorrhiza

Organism

Organism	UniProt	Comment	Textmining
Salvia miltiorrhiza	-	-	-

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Release 2023.1 (January 2023)