Literature summary for 4.2.3.114 extracted from

Bromann, K.; Toivari, M.; Viljanen, K.; Ruohonen, L.; Nakari-Setaelae, T.
Engineering Aspergillus nidulans for heterologous ent-kaurene and gamma-terpinene production (2016), Appl. Microbiol. Biotechnol., 100, 6345-6359 .

Search for more literature for 4.2.3.114

Application

Application	Comment	Organism
synthesis	recombinant expression in Aspergillus nidulans resulting in production of gamma-terpinene. Aspergillus nidulans is capable of heterologous terpene production and thus has potential as a production host for industrially relevant compounds, e.g. gamma-terpinene	Citrus unshiu

Cloned(Commentary)

Cloned (Comment)	Organism
gene CitMTSL61, HA-tag and a start codon are introduced to the synthetic gene before the arginine pair at position R56-R57 by using PCR to create gTerpS, recombinant overexpression of the codon-optimized gTerpS from plasmid pHHO5 in Aspergillus nidulans strain A772 resulting in production of gamma-terpinene, and expression of codon-optimized gTerpS in Saccharomyces cerevisiae strain H2802 from vector B1181. Real-time quantitative reverse transcription PCR expression analysis. Aspergillus nidulans is capable of heterologous terpene production and thus has potential as a production host for industrially relevant compounds	Citrus unshiu

Cloned (Comment)

Organism

gene CitMTSL61, HA-tag and a start codon are introduced to the synthetic gene before the arginine pair at position R56-R57 by using PCR to create gTerpS, recombinant overexpression of the codon-optimized gTerpS from plasmid pHHO5 in Aspergillus nidulans strain A772 resulting in production of gamma-terpinene, and expression of codon-optimized gTerpS in Saccharomyces cerevisiae strain H2802 from vector B1181. Real-time quantitative reverse transcription PCR expression analysis. Aspergillus nidulans is capable of heterologous terpene production and thus has potential as a production host for industrially relevant compounds

Citrus unshiu

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Citrus unshiu

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
geranyl diphosphate	Citrus unshiu	-	gamma-terpinene + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Citrus unshiu	Q6F5H0	i.e. Citrus nobilis var. unshiu	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
geranyl diphosphate	-	Citrus unshiu	gamma-terpinene + diphosphate	-	?
additional information	product identification and quantification by GC-MS analysis	Citrus unshiu	?	-	?

Synonyms

Synonyms	Comment	Organism
CitMTSL61	-	Citrus unshiu

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Citrus unshiu

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.6	-	assay at	Citrus unshiu