BRENDA - Enzyme Database show
show all sequences of 4.2.3.104

Isolation and characterization of terpene synthases in cotton (Gossypium hirsutum)

Yang, C.Q.; Wu, X.M.; Ruan, J.X.; Hu, W.L.; Mao, Y.B.; Chen, X.Y.; Wang, L.J.; Phytochemistry 96, 46-56 (2013)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0289
-
(2E,6E)-farnesyl diphosphate
30C, pH not specified in the publication
Gossypium hirsutum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Gossypium hirsutum
K7PRF2
isoform TPS1
-
Gossypium hirsutum
U5N1F1
isoform TPS2
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cotyledon
lowest expression level
Gossypium hirsutum
-
hypocotyl
-
Gossypium hirsutum
-
leaf
; high expression
Gossypium hirsutum
-
additional information
expression is hardly detectable in other organs than leaf and pericarp
Gossypium hirsutum
-
pericarp
highest expression level; low expression
Gossypium hirsutum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2E,6E)-farnesyl diphosphate
-
730575
Gossypium hirsutum
alpha-humulene + diphosphate
enzyme produces beta-caryophyllene and alpha-humulene in a ratio of 71:29
-
-
?
4 (2E,6E)-farnesyl diphosphate
-
730575
Gossypium hirsutum
guaia-1(10),11-diene + guaia-1(5),11-diene + alpha-humulene + beta-elemene + 4 diphosphate
products are 55% guaia-1(10),11-diene, 14% guaia-1(5),11-diene, 10% alpha-humulene, 10% beta-elemene, 6% alloaromadendrene and 5% beta-caryophyllene
-
-
?
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0289
-
(2E,6E)-farnesyl diphosphate
30C, pH not specified in the publication
Gossypium hirsutum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cotyledon
lowest expression level
Gossypium hirsutum
-
hypocotyl
-
Gossypium hirsutum
-
leaf
-
Gossypium hirsutum
-
leaf
high expression
Gossypium hirsutum
-
additional information
expression is hardly detectable in other organs than leaf and pericarp
Gossypium hirsutum
-
pericarp
highest expression level
Gossypium hirsutum
-
pericarp
low expression
Gossypium hirsutum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2E,6E)-farnesyl diphosphate
-
730575
Gossypium hirsutum
alpha-humulene + diphosphate
enzyme produces beta-caryophyllene and alpha-humulene in a ratio of 71:29
-
-
?
4 (2E,6E)-farnesyl diphosphate
-
730575
Gossypium hirsutum
guaia-1(10),11-diene + guaia-1(5),11-diene + alpha-humulene + beta-elemene + 4 diphosphate
products are 55% guaia-1(10),11-diene, 14% guaia-1(5),11-diene, 10% alpha-humulene, 10% beta-elemene, 6% alloaromadendrene and 5% beta-caryophyllene
-
-
?
Expression
Organism
Commentary
Expression
Gossypium hirsutum
expression is induced upon wounding or methyljasmonate treatment; expression is induced upon wounding or methyljasmonate treatment
up
General Information
General Information
Commentary
Organism
physiological function
after tobacco rattlevirus-based virus induced gene silencing, beta-elemene, guaia-1(5),11-diene and guaia-1(10),11-diene are undetectable in suppressed leaves
Gossypium hirsutum
General Information (protein specific)
General Information
Commentary
Organism
physiological function
after tobacco rattlevirus-based virus induced gene silencing, beta-elemene, guaia-1(5),11-diene and guaia-1(10),11-diene are undetectable in suppressed leaves
Gossypium hirsutum
Expression (protein specific)
Organism
Commentary
Expression
Gossypium hirsutum
expression is induced upon wounding or methyljasmonate treatment
up
Other publictions for EC 4.2.3.104
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746751
Krieg
CO2 to terpenes autotrophic a ...
Cupriavidus necator
Angew. Chem. Int. Ed. Engl.
57
1879-1882
2018
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1
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1
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1
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748561
Zhang
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Zingiber zerumbet
Metab. Eng.
49
28-35
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1
-
1
-
-
-
-
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1
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1
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747656
Alemdar
-
Bioproduction of alpha-humule ...
Zingiber zerumbet
Eng. Life Sci.
17
900-907
2017
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1
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4
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1
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1
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4
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3
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4
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1
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3
1
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1
1
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746789
Alemdar
Heterologous expression, puri ...
Zingiber zerumbet
Appl. Biochem. Biotechnol.
178
474-489
2016
-
-
1
-
1
-
-
2
-
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1
-
1
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1
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-
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2
1
1
1
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1
1
1
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2
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1
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1
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2
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1
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1
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2
1
1
1
-
1
1
1
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2
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2
2
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748440
Kumeta
Characterization of alpha-hum ...
Aquilaria crassna
J. Nat. Med.
70
452-459
2016
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1
-
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1
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3
-
3
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1
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6
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3
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3
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3
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6
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1
2
6
3
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-
748552
Sonntag
Engineering Methylobacterium ...
Zingiber zerumbet
Metab. Eng.
32
82-94
2015
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-
1
-
1
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1
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1
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1
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-
730575
Yang
Isolation and characterization ...
Gossypium hirsutum
Phytochemistry
96
46-56
2013
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
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5
-
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2
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1
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7
-
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2
-
-
-
-
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-
-
-
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1
1
1
2
-
-
716562
Bleeker
RNA-seq discovery, functional ...
Solanum habrochaites
Plant Mol. Biol.
77
323-336
2011
-
-
-
-
-
-
-
-
-
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1
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1
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1
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1
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1
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-
717468
Yu
Zingiber zerumbet CYP71BA1 cat ...
Zingiber zerumbet
Cell. Mol. Life Sci.
68
1033-1040
2011
-
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1
-
-
-
-
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1
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3
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3
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1
-
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1
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-
716459
Koepke
Species-specific responses of ...
Pinus sylvestris
Phytochemistry
71
909-917
2010
-
-
-
-
-
-
-
-
-
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-
8
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1
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1
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-
718233
Schilmiller
Studies of a biochemical facto ...
Solanum lycopersicum
Plant Physiol.
153
1212-1223
2010
-
-
1
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-
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-
-
-
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1
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1
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1
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1
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1
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1
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1
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-
690593
Harada
Efficient synthesis of functio ...
Zingiber zerumbet
Appl. Microbiol. Biotechnol.
81
915-925
2008
-
-
1
-
-
-
-
-
-
-
-
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6
-
-
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-
-
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1
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1
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1
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1
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-
718244
Yu
Molecular cloning and function ...
Zingiber zerumbet
Planta
227
1291-1299
2008
-
-
1
-
-
-
-
-
-
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1
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3
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1
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3
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1
1
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1
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1
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1
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1
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3
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1
1
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1
1
1
1
1
-
-
713266
van Der Hoeven
Genetic control and evolution ...
no activity in Solanum habrochaites, Solanum lycopersicum
Plant Cell
12
2283-2294
2000
-
-
-
-
-
-
-
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1
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4
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1
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1
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1
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1
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