BRENDA - Enzyme Database
show all sequences of 4.2.2.6

Performance of selected microbial pectinases on synthetic monomethyl-esterified di- and trigalacturonates

Kester, H.C.M.; Magaud, D.; Roy, C.; Anker, D.; Doutheau, A.; Shevchik, V.; Hugouvieux-Cotte-Pattat, N.; Benen, J.A.E.; Visser, J.; J. Biol. Chem. 274, 37053-37059 (1999) View publication on PubMed

Data extracted from this reference:

Organism
Organism
UniProt
Commentary
Textmining
Dickeya chrysanthemi
-
-
-
Reaction
Reaction
Commentary
Organism
Reaction ID
4-(4-deoxy-alpha-D-galact-4-enuronosyl)-D-galacturonate = 2 5-dehydro-4-deoxy-D-glucuronate
also catalyses eliminative removal of unsaturated terminal residues from oligosaccharides of D-galacturonate
Dickeya chrysanthemi
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
substrate specificity
Dickeya chrysanthemi
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1-methyl-di-D-galacturonate
alpha-(1-4)-linked
651970
Dickeya chrysanthemi
?
-
651970
Dickeya chrysanthemi
?
1-methyl-tri-D-galacturonate
alpha-(1-4)-linked
651970
Dickeya chrysanthemi
?
-
651970
Dickeya chrysanthemi
?
3-methyl-tri-D-galacturonate
best substrate, alpha-(1-4)-linked
651970
Dickeya chrysanthemi
?
-
651970
Dickeya chrysanthemi
?
DELTA4,5-unsaturated di-D-galacturonate
alpha-(1-4)-linked
651970
Dickeya chrysanthemi
?
-
651970
Dickeya chrysanthemi
?
DELTA4,5-unsaturated tri-D-galacturonate
alpha-(1-4)-linked
651970
Dickeya chrysanthemi
?
-
651970
Dickeya chrysanthemi
?
di-D-galacturonate
-
651970
Dickeya chrysanthemi
?
-
651970
Dickeya chrysanthemi
?
additional information
no activity with 2-methyl-di-G-galacturonate and 2-methyl-tri-G-galacturonate
651970
Dickeya chrysanthemi
?
-
651970
Dickeya chrysanthemi
?
tri-D-galacturonate
-
651970
Dickeya chrysanthemi
?
-
651970
Dickeya chrysanthemi
?
Synonyms
Synonyms
Commentary
Organism
Ogl
-
Dickeya chrysanthemi
oligogalacturonan lyase
-
Dickeya chrysanthemi
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Dickeya chrysanthemi
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Dickeya chrysanthemi
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
substrate specificity
Dickeya chrysanthemi
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1-methyl-di-D-galacturonate
alpha-(1-4)-linked
651970
Dickeya chrysanthemi
?
-
651970
Dickeya chrysanthemi
?
1-methyl-tri-D-galacturonate
alpha-(1-4)-linked
651970
Dickeya chrysanthemi
?
-
651970
Dickeya chrysanthemi
?
3-methyl-tri-D-galacturonate
best substrate, alpha-(1-4)-linked
651970
Dickeya chrysanthemi
?
-
651970
Dickeya chrysanthemi
?
DELTA4,5-unsaturated di-D-galacturonate
alpha-(1-4)-linked
651970
Dickeya chrysanthemi
?
-
651970
Dickeya chrysanthemi
?
DELTA4,5-unsaturated tri-D-galacturonate
alpha-(1-4)-linked
651970
Dickeya chrysanthemi
?
-
651970
Dickeya chrysanthemi
?
di-D-galacturonate
-
651970
Dickeya chrysanthemi
?
-
651970
Dickeya chrysanthemi
?
additional information
no activity with 2-methyl-di-G-galacturonate and 2-methyl-tri-G-galacturonate
651970
Dickeya chrysanthemi
?
-
651970
Dickeya chrysanthemi
?
tri-D-galacturonate
-
651970
Dickeya chrysanthemi
?
-
651970
Dickeya chrysanthemi
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Dickeya chrysanthemi
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Dickeya chrysanthemi
Other publictions for EC 4.2.2.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
715562
Abbott
The active site of oligogalact ...
Yersinia enterocolitica, Yersinia enterocolitica 8081
J. Biol. Chem.
285
39029-39038
2010
-
-
1
1
-
-
1
1
-
1
-
2
-
5
-
-
1
1
-
-
-
-
2
1
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
1
-
1
-
2
-
-
-
1
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
677684
Yang
Global effect of indole-3-acet ...
Dickeya chrysanthemi
Appl. Environ. Microbiol.
73
1079-1088
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
34108
Shevchik
The exopolygalacturonate lyase ...
Dickeya chrysanthemi, Dickeya chrysanthemi 3937
J. Bacteriol.
181
3912-3919
1999
-
-
1
-
-
-
1
2
2
4
1
-
-
14
-
-
-
-
-
-
-
-
6
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
2
2
4
1
-
-
-
-
-
-
-
-
-
6
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
651970
Kester
Performance of selected microb ...
Dickeya chrysanthemi
J. Biol. Chem.
274
37053-37059
1999
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
1
-
-
1
-
8
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
8
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
34109
Reverchon
Nucleotide sequences of the Er ...
Dickeya chrysanthemi, Dickeya chrysanthemi 3937
Gene
85
125-134
1989
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
34107
Reverchon
Molecular cloning of an Erwini ...
Dickeya chrysanthemi, Dickeya chrysanthemi 3937
Gene
55
125-133
1987
-
-
1
-
-
-
-
-
-
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
34106
Hatanaka
-
Pectolytic enzymes of exo-type ...
Pseudomonas sp.
Agric. Biol. Chem.
35
1617-1624
1971
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
34104
Hatanaka
-
An oligogalacturonate transeli ...
Erwinia aroidea
Agric. Biol. Chem.
34
1618-1624
1970
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
7
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
34105
Moran
Oligogalacturonide trans-elimi ...
Erwinia aroidea
Arch. Biochem. Biophys.
125
734-741
1968
-
-
-
-
-
-
-
2
-
-
-
1
-
3
-
-
1
-
-
-
1
-
4
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
1
-
-
1
-
4
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-