BRENDA - Enzyme Database
show all sequences of 4.2.2.6

The exopolygalacturonate lyase PelW and the oligogalacturonate lyase Ogl, two cytoplasmic enzymes of pectin catabolism in Erwinia chrysanthemi 3937

Shevchik, V.E.; Condemine, G.; Robert-Baudouy, J.; Hugouvieux-Cotte-Pattat, N.; J. Bacteriol. 181, 3912-3919 (1999) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
-
Dickeya chrysanthemi
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
-
Dickeya chrysanthemi
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.12
-
trigalacturonic acid
-
Dickeya chrysanthemi
0.15
-
digalacturonic acid
-
Dickeya chrysanthemi
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Dickeya chrysanthemi
5737
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
strong activation
Dickeya chrysanthemi
Fe2+
strong activation
Dickeya chrysanthemi
Mn2+
best cofactor, optimal concentration: 0.1 mM
Dickeya chrysanthemi
Ni2+
strong activation
Dickeya chrysanthemi
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44124
-
x * 44124, calculation from nucleotide sequence
Dickeya chrysanthemi
Organism
Organism
UniProt
Commentary
Textmining
Dickeya chrysanthemi
-
3937
-
Dickeya chrysanthemi 3937
-
3937
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
digalacturonic acid
-
34108
Dickeya chrysanthemi
?
-
-
-
?
digalacturonic acid
-
34108
Dickeya chrysanthemi 3937
?
-
-
-
?
tetragalacturonic acid
-
34108
Dickeya chrysanthemi
?
-
-
-
?
tetragalacturonic acid
-
34108
Dickeya chrysanthemi 3937
?
-
-
-
?
trigalacturonic acid
-
34108
Dickeya chrysanthemi
?
-
-
-
?
trigalacturonic acid
-
34108
Dickeya chrysanthemi 3937
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 44124, calculation from nucleotide sequence
Dickeya chrysanthemi
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
7.7
-
Dickeya chrysanthemi
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Dickeya chrysanthemi
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
-
Dickeya chrysanthemi
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.12
-
trigalacturonic acid
-
Dickeya chrysanthemi
0.15
-
digalacturonic acid
-
Dickeya chrysanthemi
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Dickeya chrysanthemi
5737
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
strong activation
Dickeya chrysanthemi
Fe2+
strong activation
Dickeya chrysanthemi
Mn2+
best cofactor, optimal concentration: 0.1 mM
Dickeya chrysanthemi
Ni2+
strong activation
Dickeya chrysanthemi
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44124
-
x * 44124, calculation from nucleotide sequence
Dickeya chrysanthemi
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
digalacturonic acid
-
34108
Dickeya chrysanthemi
?
-
-
-
?
digalacturonic acid
-
34108
Dickeya chrysanthemi 3937
?
-
-
-
?
tetragalacturonic acid
-
34108
Dickeya chrysanthemi
?
-
-
-
?
tetragalacturonic acid
-
34108
Dickeya chrysanthemi 3937
?
-
-
-
?
trigalacturonic acid
-
34108
Dickeya chrysanthemi
?
-
-
-
?
trigalacturonic acid
-
34108
Dickeya chrysanthemi 3937
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 44124, calculation from nucleotide sequence
Dickeya chrysanthemi
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
7.7
-
Dickeya chrysanthemi
Other publictions for EC 4.2.2.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
715562
Abbott
The active site of oligogalact ...
Yersinia enterocolitica, Yersinia enterocolitica 8081
J. Biol. Chem.
285
39029-39038
2010
-
-
1
1
-
-
1
1
-
1
-
2
-
5
-
-
1
1
-
-
-
-
2
1
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
1
-
1
-
2
-
-
-
1
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
677684
Yang
Global effect of indole-3-acet ...
Dickeya chrysanthemi
Appl. Environ. Microbiol.
73
1079-1088
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
34108
Shevchik
The exopolygalacturonate lyase ...
Dickeya chrysanthemi, Dickeya chrysanthemi 3937
J. Bacteriol.
181
3912-3919
1999
-
-
1
-
-
-
1
2
2
4
1
-
-
14
-
-
-
-
-
-
-
-
6
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
2
2
4
1
-
-
-
-
-
-
-
-
-
6
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
651970
Kester
Performance of selected microb ...
Dickeya chrysanthemi
J. Biol. Chem.
274
37053-37059
1999
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
1
-
-
1
-
8
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
8
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
34109
Reverchon
Nucleotide sequences of the Er ...
Dickeya chrysanthemi, Dickeya chrysanthemi 3937
Gene
85
125-134
1989
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
34107
Reverchon
Molecular cloning of an Erwini ...
Dickeya chrysanthemi, Dickeya chrysanthemi 3937
Gene
55
125-133
1987
-
-
1
-
-
-
-
-
-
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
34106
Hatanaka
-
Pectolytic enzymes of exo-type ...
Pseudomonas sp.
Agric. Biol. Chem.
35
1617-1624
1971
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
34104
Hatanaka
-
An oligogalacturonate transeli ...
Erwinia aroidea
Agric. Biol. Chem.
34
1618-1624
1970
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
7
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
34105
Moran
Oligogalacturonide trans-elimi ...
Erwinia aroidea
Arch. Biochem. Biophys.
125
734-741
1968
-
-
-
-
-
-
-
2
-
-
-
1
-
3
-
-
1
-
-
-
1
-
4
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
1
-
-
1
-
4
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-