Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Flammeovirga sp. MY04 |
Protein Variants | Comment | Organism |
---|---|---|
T282N | deletion of the noncatalytic region, 1.5fold increase in specific activity compared to wild-type | Flammeovirga sp. MY04 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
alginate | Km value of wild-type 0.135 mg/ml, of mutant T282N 0.158 mg/ml, pH 6.0, 40°C | Flammeovirga sp. MY04 | |
additional information | - |
poly(alpha-(1,4)-L-guluronate) | Km value of wild-type 0.706 mg/ml, of mutant T282N 1.224 mg/ml, pH 6.0, 40°C | Flammeovirga sp. MY04 | |
additional information | - |
poly(beta-(1,4)-D-mannuronate) | Km value of wild-type 2.845 mg/ml, of mutant T282N 0.392 mg/ml, pH 6.0, 40°C | Flammeovirga sp. MY04 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flammeovirga sp. MY04 | A0A1B1FUS6 | - |
- |
Purification (Comment) | Organism |
---|---|
purification using Ni-NTA column | Flammeovirga sp. MY04 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2025 | - |
wild-type, pH 6.0, 40°C | Flammeovirga sp. MY04 |
2920 | - |
mutant T282N, pH 6.0, 40°C | Flammeovirga sp. MY04 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alginate | - |
Flammeovirga sp. MY04 | unsaturated algino-oligosaccharides | - |
? | |
poly(alpha-(1,4)-L-guluronate) | reaction of EC 4.2.2.11, about 15% of the activity with poly(beta-(1,4)-D-mannuronate) | Flammeovirga sp. MY04 | 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1->4)-alpha-oligo(alpha-(1->4)-L-guluronate) | - |
? | |
poly(beta-(1,4)-D-mannuronate) | - |
Flammeovirga sp. MY04 | 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1->4)-beta-oligo(beta-(1->4)-D-mannuronate) | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60580, calculated from sequence | Flammeovirga sp. MY04 |
Synonyms | Comment | Organism |
---|---|---|
Aly2 | - |
Flammeovirga sp. MY04 |
MY04_2544 | - |
Flammeovirga sp. MY04 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
wild-type, 2 h, less than 30% residual activity. Mutant T282N, 8 h, 50% residual activity | Flammeovirga sp. MY04 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Flammeovirga sp. MY04 | calculated from sequence | - |
9.1 |