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Literature summary for 4.2.2.3 extracted from
- cDNA cloning and bacterial expression of a PL-14 alginate lyase from a herbivorous marine snail Littorina brevicula (2012), Carbohydr. Res., 360, 69-77.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Littorina brevicula |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K94A | site-directed mutagenesis, the enzyme shows reduced activity compared to the wild-type enzyme | Littorina brevicula |
| K97A | site-directed mutagenesis, the enzyme shows reduced activity compared to the wild-type enzyme | Littorina brevicula |
| R123A | site-directed mutagenesis, the enzyme shows reduced activity compared to the wild-type enzyme | Littorina brevicula |
| T121A | site-directed mutagenesis, the enzyme shows reduced activity compared to the wild-type enzyme, the replacement of T121 by Ala changes the substrate preference of LbAly28 | Littorina brevicula |
| Y135A | site-directed mutagenesis, the enzyme shows reduced activity compared to the wild-type enzyme | Littorina brevicula |
| Y137A | site-directed mutagenesis, the enzyme shows reduced activity compared to the wild-type enzyme | Littorina brevicula |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
x * 28000, recombinant His-tagged wild-type enzyme, SDS-PAGE, x * 28999, sequence calculation | Littorina brevicula |
| 28999 | - |
x * 28000, recombinant His-tagged wild-type enzyme, SDS-PAGE, x * 28999, sequence calculation | Littorina brevicula |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Littorina brevicula | the enzyme is an endolytic polymannuronate lyase | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Littorina brevicula | G9MBE4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Littorina brevicula |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is an endolytic polymannuronate lyase | Littorina brevicula | ? | - |
? | |
| poly(beta-D-mannuronate) | the region including T121 of LbAly28 is the recognition of poly(MG) region of alginate | Littorina brevicula | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 28000, recombinant His-tagged wild-type enzyme, SDS-PAGE, x * 28999, sequence calculation | Littorina brevicula |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alginate lyase | - |
Littorina brevicula |
| LbAly28 | - |
Littorina brevicula |
| poly(M) lyase | - |
Littorina brevicula |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Littorina brevicula |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Littorina brevicula |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the family PL-14 | Littorina brevicula |
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