Application | Comment | Organism |
---|---|---|
medicine | alginate lyases are promising therapeutic candidates for treating mucoid Pseudomonas aeruginosa infections. In particular, the enzymes' ability to degrade alginate, a key component of mucoid biofilm matrix, has been the presumed mechanism by which they disrupt biofilms and enhance antibiotic efficacy, but invitro modeling shows that alginate lyase dispersion of Pseudomonas aeruginosa biofilms and enzyme synergy with tobramycin are completely decoupled from catalytic activity | Pseudomonas aeruginosa |
medicine | alginate lyases are promising therapeutic candidates for treating mucoid Pseudomonas aeruginosa infections. In particular, the enzymes' ability to degrade alginate, a key component of mucoid biofilm matrix, has been the presumed mechanism by which they disrupt biofilms and enhance antibiotic efficacy, but invitro modeling shows that alginate lyase dispersion of Pseudomonas aeruginosa biofilms and enzyme synergy with tobramycin are completely decoupled from catalytic activity | Sphingomonas sp. |
Cloned (Comment) | Organism |
---|---|
expression of C-terminally His6-tagged A1-III | Sphingomonas sp. |
expression of C-terminally His6-tagged AlgL | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
H188A | site-directed mutagenesis, the mutation switches the histidine 188 GTG codon to a CGC codon | Sphingomonas sp. |
Y242F | site-directed mutagenesis, the mutation replaces the tyrosine 242 GTA codon with a GAA codon | Sphingomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | pseudo Michaelis-Menten kinetics of native and recombinant His-tagged enzymes, overview | Pseudomonas aeruginosa | |
additional information | - |
additional information | pseudo Michaelis-Menten kinetics of native and recombinant His-tagged enzymes, overview | Sphingomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q06749 | gene algL | - |
Sphingomonas sp. | Q75WP3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged A1-III by nickel affinity chromatography and dialysis, native enzyme by hydroxyapatite chromatography, ultrafiltration, and gel filtration | Sphingomonas sp. |
recombinant C-terminally His6-tagged AlgL by nickel affinity chromatography and dialysis, native enzyme by hydroxyapatite chromatography, ultrafiltration, and gel filtration | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrates are medium-viscosity brown seaweed alginate (BSWA) and bacterial (FRD1) alginate substrates from mucoid FRD1 biofilms | Pseudomonas aeruginosa | ? | - |
? | |
additional information | substrates are medium-viscosity brown seaweed alginate (BSWA) and bacterial (FRD1) alginate substrates from mucoid FRD1 biofilms | Sphingomonas sp. | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
A1-III | - |
Sphingomonas sp. |
alginate lyase | - |
Pseudomonas aeruginosa |
alginate lyase | - |
Sphingomonas sp. |
AlgL | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pseudomonas aeruginosa |
37 | - |
assay at | Sphingomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Pseudomonas aeruginosa |
7 | - |
assay at | Sphingomonas sp. |
General Information | Comment | Organism |
---|---|---|
metabolism | alginate lyase dispersion of Pseudomonas aeruginosa biofilms and enzyme synergy with tobramycin are completely decoupled from catalytic activity, in vitro modeling, overview | Pseudomonas aeruginosa |
metabolism | alginate lyase dispersion of Pseudomonas aeruginosa biofilms and enzyme synergy with tobramycin are completely decoupled from catalytic activity, in vitro modeling, overview | Sphingomonas sp. |