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Literature summary for 4.2.2.3 extracted from

  • Xiao, L.; Han, F.; Yang, Z.; Lu, X.; Yu, W.
    A novel alginate lyase with high activity on acetylated alginate of Pseudomonas aeruginosa FRD1 from Pseudomonas sp. QD03 (2006), World J. Microbiol. Biotechnol., 22, 81-88.
    View publication on PubMed
Search for more literature for 4.2.2.3

Application

Application Comment Organism
medicine it might be possible to use alginate lyase AlgL as an adjuvant therapeutic medicine for the treatment of disease associated with Pseudomonas aeruginosa infection Pseudomonas sp.

Cloned(Commentary)

Cloned (Comment) Organism
algL is expressed in pET24a (+)/E. coli BL21 (DE3) system Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, 18% inhibition Pseudomonas sp.
Fe3+ 1 mM, complete inhibition Pseudomonas sp.
Ni2+ 1 mM, 27% inhibition Pseudomonas sp.
SDS 1 mM, complete inhibition Pseudomonas sp.
Zn2+ 1 mM, 21% inhibition Pseudomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Pseudomonas sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 1 mM, 4.2fold increase in activity Pseudomonas sp.
Ca2+ 1 mM, 2.5fold increase in activity Pseudomonas sp.
K+ 1 mM, 4.2fold increase in activity Pseudomonas sp.
Mg2+ 1 mM, 3.1fold increase in activity Pseudomonas sp.
Mn2+ 1 mM, 3.2fold increase in activity Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42200
-
 x * 42200, calculated from sequence Pseudomonas sp.
42800
-
 x * 42800, SDS-PAGE Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. Q597B8
-
-
Pseudomonas sp. QD03 Q597B8
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate can degrade alginate and mannuronate blocks, but hardly degrades guluronate blocks. In particular, AlgL can degrade acetylated alginate of Pseudomonas aeruginosa FRD1 Pseudomonas sp. ?
-
 ?
alginate can degrade alginate and mannuronate blocks, but hardly degrades guluronate blocks. In particular, AlgL can degrade acetylated alginate of Pseudomonas aeruginosa FRD1 Pseudomonas sp. QD03 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 42800, SDS-PAGE Pseudomonas sp.
? x * 42200, calculated from sequence Pseudomonas sp.

Synonyms

Synonyms Comment Organism
AlgL
-
 Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Pseudomonas sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45 25°C: about 55% of maximal activity, 45°C: about 65% of maximal activity Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 1 h, about 10% loss of activity Pseudomonas sp.
40
-
 1 h, about 20% loss of activity Pseudomonas sp.
50
-
 1 h, about 40% loss of activity Pseudomonas sp.
60
-
 1 h, about 75% loss of activity Pseudomonas sp.
80
-
 1 h, complete loss of activity Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8 pH 6.5: about 45% of maximal activity, pH 8.0: about 50% of maximal activity Pseudomonas sp.