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Literature summary for 4.2.2.22 extracted from
- Cloning of the pelA gene from Bacillus licheniformis 14A and biochemical characterization of recombinant, thermostable, high-alkaline pectate lyase (2004), Appl. Microbiol. Biotechnol., 64, 560-567.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli as a recombinant fusion protein containing a C-terminal His-tag | Bacillus licheniformis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| polygalacturonic acid | recombinant fusion protein containing a C-terminal His-tag is inhibited by excess substrate | Bacillus licheniformis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km-value for polygalacturonic acid is 0.56 g/l, recombinant fusion protein containing a C-terminal His-tag | Bacillus licheniformis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33451 | - |
x * 33451, calculated from sequence | Bacillus licheniformis |
| 36000 | - |
x * 36000, SDS-PAGE | Bacillus licheniformis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | Q8GCB2 | - |
- |
| Bacillus licheniformis 14A | Q8GCB2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant | Bacillus licheniformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polygalacturonic acid | - |
Bacillus licheniformis | unsaturated trigalacturonate | main product | ? | |
| polygalacturonic acid | - |
Bacillus licheniformis 14A | unsaturated trigalacturonate | main product | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 36000, SDS-PAGE | Bacillus licheniformis |
| ? | x * 33451, calculated from sequence | Bacillus licheniformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
recombinant fusion protein containing a C-terminal His-tag | Bacillus licheniformis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Ki-value for polygalacturonic acid is 1.08 g/l | Bacillus licheniformis |
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