General Stability | Organism |
---|---|
the enzyme catalytic activity and intrinsic fluorescence intensity increase in the presence of cosolvents glycerol, sorbitol and trehalose whereas no considerable conformational changes are observed in far-UV CD spectra. In the presence of trehalose a significant increase in meltin temperature is observed. | Proteus vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
chondroitin 4-sulfate | presence of 1 M sorbitol, pH 6.8, 37°C | Proteus vulgaris | |
0.035 | - |
chondroitin 4-sulfate | presence of 1 M trehalose, pH 6.8, 37°C | Proteus vulgaris | |
0.039 | - |
chondroitin 4-sulfate | presence of 20% glycerol, pH 6.8, 37°C | Proteus vulgaris | |
0.044 | - |
chondroitin 4-sulfate | pH 6.8, 37°C | Proteus vulgaris | |
0.055 | - |
chondroitin 6-sulfate | pH 6.8, 37°C | Proteus vulgaris | |
0.094 | - |
dermatan sulfate | pH 6.8, 37°C | Proteus vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Proteus vulgaris | P59807 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Storage Stability | Organism |
---|---|
-18°C, 3 days, complete loss of activity | Proteus vulgaris |
25°C, 17 days, complete loss of activity, in presence of 1 M trehalose 85% residual activity after 21 days | Proteus vulgaris |
37°C, 10 days, presence of 1 M trehalose, 20% residual activity | Proteus vulgaris |
4°C, 21 days, 80% residual activity | Proteus vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chondroitin 4-sulfate | - |
Proteus vulgaris | ? | - |
? | |
chondroitin 6-sulfate | - |
Proteus vulgaris | ? | - |
? | |
dermatan sulfate | - |
Proteus vulgaris | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Proteus vulgaris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
47 | - |
melting temperature | Proteus vulgaris |
49 | - |
melting temperature, presence of 20% glycerol | Proteus vulgaris |
52 | - |
melting temperature, presence of 1 M sorbitol | Proteus vulgaris |
54 | - |
melting temperature, presence of 1 M trehalose | Proteus vulgaris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
48.5 | - |
dermatan sulfate | pH 6.8, 37°C | Proteus vulgaris | |
69.7 | - |
chondroitin 4-sulfate | presence of 1 M sorbitol, pH 6.8, 37°C | Proteus vulgaris | |
75.8 | - |
chondroitin 4-sulfate | presence of 1 M trehalose, pH 6.8, 37°C | Proteus vulgaris | |
78.8 | - |
chondroitin 4-sulfate | presence of 20% glycerol, pH 6.8, 37°C | Proteus vulgaris | |
84.8 | - |
chondroitin 4-sulfate | pH 6.8, 37°C | Proteus vulgaris | |
100 | - |
chondroitin 6-sulfate | pH 6.8, 37°C | Proteus vulgaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Proteus vulgaris |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1517 | - |
dermatan sulfate | pH 6.8, 37°C | Proteus vulgaris | |
1800 | - |
chondroitin 6-sulfate | pH 6.8, 37°C | Proteus vulgaris | |
1933 | - |
chondroitin 4-sulfate | pH 6.8, 37°C | Proteus vulgaris | |
2017 | - |
chondroitin 4-sulfate | presence of 20% glycerol, pH 6.8, 37°C | Proteus vulgaris | |
2117 | - |
chondroitin 4-sulfate | presence of 1 M sorbitol, pH 6.8, 37°C | Proteus vulgaris | |
2167 | - |
chondroitin 4-sulfate | presence of 1 M trehalose, pH 6.8, 37°C | Proteus vulgaris |