Cloned (Comment) | Organism |
---|---|
gene Bsu11286Pel, DNA and amino acid sequence determination and analysis, recombinant expression in Bacillus subtilis strain WB600 | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38600 | - |
x * 45498, sequence calculation, x * 38600, mature recombinant enzyme, SDS-PAGE | Bacillus subtilis |
45498 | - |
x * 45498, sequence calculation, x * 38600, mature recombinant enzyme, SDS-PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
isolated from soil of a saline-alkaline field in Tianjin, China, gene Bsu11286Pel | - |
Bacillus subtilis TCCC11286 | - |
isolated from soil of a saline-alkaline field in Tianjin, China, gene Bsu11286Pel | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 17.3fold from strain WB600 culture supernatant by ammonium sulfate fractionation, cation exchange chromatography, and gel filtration | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
444.6 | - |
purified recombinant enzyme, pH 9.0, 50°C | Bacillus subtilis |
Subunits | Comment | Organism |
---|---|---|
? | x * 45498, sequence calculation, x * 38600, mature recombinant enzyme, SDS-PAGE | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
alkaline pectate lyase | - |
Bacillus subtilis |
Apel | - |
Bacillus subtilis |
pectate transeliminase | - |
Bacillus subtilis |
Pel | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | activity range | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
purified recombinant enzyme, pH 9.0, 2 h, over 90% activity remaining | Bacillus subtilis |
40 | - |
purified recombinant enzyme, pH 9.0, 2 h, over 80% activity remaining | Bacillus subtilis |
50 | - |
purified recombinant enzyme, 2 h, 90% activity remaining | Bacillus subtilis |
60 | - |
purified recombinant enzyme, pH 9.0, half-life is 20 min, inactivation after 80 min | Bacillus subtilis |
70 | - |
purified recombinant enzyme, pH 9.0, half-life is about 12 min, inactivation after 60 min | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 11 | activity range | Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 11.5 | purified recombinant enzyme, 2 h, stable up to pH 9.0, inactivation at pH 11.5 | Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
evolution | the mature Apel is structurally related to the enzymes in the polysaccharide lyase family 1 | Bacillus subtilis |
additional information | Pel structure modeling, overview | Bacillus subtilis |